Canine Mesenchymal Stem Cells

(CnMSC)

Canine Mesenchymal Stem Cells (CnMSC) are derived from canine bone marrow and are cryopreserved at second passage. CnMSC can be propagated 10 population doublings. In addition to supporting hematopoietic cells^1,2, mesenchymal stem cells can be induced to differentiate into cells of various tissues, including bone^3,4,5,6, podocytes⁷, smooth muscle cells^8,9, and neural cells¹⁰. The multipotent potential of CnMSC provides new avenues for cell-based therapy^11,12.

1. Al-Lebban, ZS et al., Can J Vet Res. 51(2):162 (1987).

2. Neuner, E et al., Vet Immunol Immunopathol. 61(1):1 (1998).

3. Kadiyala, S et al., Cell Transplant. 6(2):125 (1997).

4. Yuan, J et al., Biomaterials. 28(6):1005 (2007).

5. Mankani, MH et al., Am J Pathol. 168(2):542 (2006).

6. Volk, SW et al., Am J Vet Res. 66(10):1729 (2005).

7. Perry, J et al., J Am Soc Nephrol 17(1):66 (2006).

8. Zhang, Y et al., BJU Int. 96(7):1120 (2005).

9. Cho, SW et al., Biomaterials 25(15):2979 (2004).

10. Kamishina, H et al., Am J Vet Res. 67(11):1921 (2006).

11. Cherington, V et al., Hum Gene Ther. 9(10):1397 (1998).

12. Chiang, GG et al., Hum Gene Ther. 10(1):61 (1999).

Each lot tested negative for HIV, Hepatitis B and Hepatitis C and negative for mycoplasma, bacteria, yeast, fungi.

Product Name	Product Description	Size (vol)	Cat No.
CnMSC Total Kit	Each kit contains an ampoule of cryopreserved CnMSC (Cn492-05), 500 ml of Canine Mesenchymal Stem Cell Growth Medium (Cn419-500), and a Subculture Reagent Kit (090K).	1 kit	Cn492K-05
Cryopreserved CnMSC	(>500,000 cells in an ampoule) Cryopreserved in ampoules at first passage in Canine Marrow Stromal Cell Basal Medium that contains 10% FBS and 10% DMSO.	1 ampoule	Cn492-05
Proliferating CnMSC	Cultured in flasks at second passage in Canine Marrow Stromal Cell Growth Medium.	T-25 flask 2 T-25 flasks T-75 flask or 3 T-25 flasks	Cn493-25 Cn493-50 Cn493-75
Proliferating CnMSC	Cultured in plates at second passage in Canine Marrow Stromal Cell Growth Medium.	6 well plate 12 well plate 24 well plate 48 well plate 96 well plate	Cn493-6w Cn493-12w Cn493-24w Cn493-48w Cn493-96w
Canine Mesenchymal Stem Cell Growth Medium	Optimized and ready for use.	500 ml	Cn419-500
Canine Mesenchymal Stem Cell Growth Medium Kit	Basal Medium & Growth Supplement packaged separately.	Yields 500ml	Cn419K-500
Canine Mesenchymal Stem Cell Basal Medium	Add Growth Supplements before use.	500 ml	Cn418-500
Canine Marrow Stromal Cell Growth Supplements		30 ml	Cn419-GS
Subculture Reagent Kit	(100 ml each of HBSS, Trypsin/EDTA & Neutralizer Solution)	100 ml	090K