Canine Osteoblasts

(CnOb)

Canine Osteoblasts (CnOb) are isolated from canine bone and are cryopreserved at second passage. CnOb can be passaged at least 10 population doublings. The dog model provides a system for the study of bone remodeling agents such as osteoblast-stimulating bone morphogenetic proteins (BMPs)^1,2 and bisphosphonates³, as well as anti-remodeling agents⁴. Canine osteoblasts have been used in prospective bone regeneration models utilzing such 3-D bioactive scaffolds as polycaprolactone⁵.

1. Schmoekel, HG et al., J Orthop Res. 22(2):376 (2004).

2. ten Dijke, P. Curr Med Res Opin. 22(S):S7 (2006).

3. Im, GI et al., Biomaterials. 25(18):4105 (2004).

4. Allen, MR et al., Calcif Tissue Int. 79(4):255 (2006).

5. Rai, B et al., Biomaterials. 25(24):5499 (2004).

Each lot tested negative for HIV, Hepatitis B and Hepatitis C and negative for mycoplasma, bacteria, yeast, fungi.

Product Name	Product Description	Size (vol)	Cat No.
CnOb Total Kit	Each kit contains an ampoule of cryopreserved CnOb (Cn406-05), 500 ml of Canine Osteoblast Growth Medium (Cn417-500), and a Subculture Reagent Kit (090K).	1 kit	Cn406K-05
Cryopreserved CnOb	(>500,000 cells in an ampoule) Cryopreserved in ampoules at first passage in Canine Osteoblast Basal Medium that contains 10% FBS and 10% DMSO.	1 ampoule	Cn406-05
Proliferating CnOb	Cultured in flasks at second passage in Canine Osteoblast Growth Medium.	T-25 flask 2 T-25 flasks T-75 flask or 3 T-25 flasks	Cn407-25 Cn407-50 Cn407-75
Proliferating CnOb	Cultured in plates at second passage in Canine Osteoblast Growth Medium.	6 well plate 12 well plate 24 well plate 48 well plate 96 well plate	Cn407-6w Cn407-12w Cn407-24w Cn407-48w Cn407-96w
Canine Osteoblast Growth Medium	Optimized and ready for use.	500 ml	Cn417-500
Canine Osteoblast Growth Medium Kit	Basal Medium & Growth Supplement packaged separately.	Yields 500ml	Cn417K-500
Canine Osteoblast Basal Medium	Add Growth Supplements before use.	500 ml	Cn416-500
Canine Osteoblast Growth Supplements		30 ml	Cn417-GS
Subculture Reagent Kit	(100 ml each of HBSS, Trypsin/EDTA & Neutralizer Solution)	100 ml	090K