Canine Skeletal Muscle Cells

(CnSkMC)

Canine Skeletal Muscle Cells (CnSkMC) are derived from the limbal skeletal muscle and are cryopreserved at second passage. CnSkMC can be passaged at least 15 population doublings. Muscle diseases can occur in dogswith clinical presentations resembling human disorders, thus the caninesystem can be a valuable intermediate disease model^1,2. The canine model has been used to study the effects of hypoxia^3,4,5, ischemia^6,7, and hemorrhagic shock⁸ on skeletal muscle. CnSkMC represent an in vitro cellular system to study the effects of diet on skeletal muscle^9,10,11.

1. Shelton, GD & Enqvall, E, Neuromuscul Disord. 15(2):127 (2005).

2. Ytterberg, SR, Curr Opin Rheumatol. 3(6):934 (1991).

3.Mo, FM & Ballard, HJ. Am J Physiol Heart Circ Physiol 289(4):H1710 (2005).

4. Mo, FM & Ballard, HJ. J Physiol. 536(2):592 (2001).

5. da Cruz, CA et al., J Cardiovasc Surg (Torino). 38(5):473 (1997).

6. Timmons, JA et al., J Clin Invest. 97(3):879 (1996).

7. Roberts, PA et al., J Physiol. 544(2):591 (2002).

8. Sunder-Plassman, L et al., Res Exp Med (Berl) 159(3):167 (1973).

9. McKenzie, E et al., Med Sci Sports Exerc. 37(8(:1307 (2005).

10. Helman, EE et al., J Anim Sci. 81(9):2199 (2003).

11. Reynolds, Aj et al., J Appl Physiol. 79(5):1601 (1995).

Each lot tested negative for HIV, Hepatitis B and Hepatitis C and negative for mycoplasma, bacteria, yeast, fungi.

Product Name	Product Description	Size (vol)	Cat No.
CnSkMC Total Kit	Each kit contains an ampoule of cryopreserved CnSkMC (Cn150-05), 500 ml of Canine Skeletal Muscle Cell Growth Medium (Cn151-500), and a Subculture Reagent Kit (090K).	1 kit	Cn150K-05
Cryopreserved CnSkMC	(>500,000 cells in an ampoule) Cryopreserved in ampoules at first passage in Canine Skeletal Muscle Cell Basal Medium that contains 10% FBS and 10% DMSO.	1 ampoule	Cn150-05
Proliferating CnSkMC	Cultured in flasks at second passage in Canine Skeletal Muscle Cell Growth Medium.	T-25 flask 2 T-25 flasks T-75 flask or 3 T-25 flasks	Cn151-25 Cn151-50 Cn151-75
Proliferating CnSkMC	Cultured in plates at second passage in Canine Skeletal Muscle Cell Growth Medium.	6 well plate 12 well plate 24 well plate 48 well plate 96 well plate	Cn151-6w Cn151-12w Cn151-24w Cn151-48w Cn151-96w
Canine Skeletal Muscle Cell Growth Medium	Optimized and ready for use.	500 ml	Cn151-500
Canine Skeletal Muscle Cell Growth Medium Kit	Basal Medium & Growth Supplement packaged separately.	Yields 500ml	Cn151K-500
Canine Skeletal Muscle Cell Basal Medium	Add Growth Supplements before use.	500 ml	Cn150-500
Canine Skeletal Muscle Cell Growth Supplements		2 x 50 ml	Cn151-GS
Subculture Reagent Kit	(100 ml each of HBSS, Trypsin/EDTA & Neutralizer Solution)	100 ml	090K