Feline Skeletal Muscle Cells

(FSkMC)

Feline Skeletal Muscle Cells (FSkMC) are isolated from the limbal skeletal muscle. They are cryopreserved at second passage and can be cultured and propagated for at least 15 population doublings. Muscle diseases can occur in cats with clinical presentations resembling human disorders, and thus the feline system can be a valuable intermediate disease model^1,2,3. ATP concentration and hypoxia have been studied in feline skeletal muscle^4,5.

1. Shelton, GD & Enqvall, E, Neuromuscul Disord. 15(2):127 (2005).

2. Ytterberg, SR, Curr Opin Rheumatol. 3(6):934 (1991).

3. Winand, NJ et al., Neuromuscul Disord. 4(5-6): 433 (1994).

4. Li, J et al., J Appl Physiol 95(2):577 (2003).

5. Nioka, S et al., Adv Exp Med Biol. 510:267 (2003).

Each lot tested negative for HIV, Hepatitis B and Hepatitis C and negative for mycoplasma, bacteria, yeast, fungi.

Product Name	Product Description	Size (vol)	Cat No.
FSkMC Total Kit	Each kit contains an ampoule of cryopreserved FSkMC (F150-05), 500 ml of Feline Skeletal Muscle Cell Growth Medium (F151-500), and a Subculture Reagent Kit (090K).	1 kit	F150K-05
Cryopreserved FSkMC	(>500,000 cells in an ampoule) Cryopreserved in ampoules at first passage in Feline Skeletal Muscle Cell Basal Medium that contains 10% FBS and 10% DMSO.	1 ampoule	F150-05
Proliferating FSkMC	Cultured in flasks at second passage in Feline Skeletal Muscle Cell Growth.	T-25 flask 2 T-25 flasks T-75 flask or 3 T-25 flasks	F151-25 F151-50 F151-75
Proliferating FSkMC	Cultured in plates at second passage in Feline Skeletal Muscle Cell Growth Medium.	6 well plate 12 well plate 24 well plate 48 well plate 96 well plate	F151-6w F151-12w F151-24w F151-48w F151-96w
Feline Skeletal Muscle Cell Growth Medium	Optimized and ready for use.	500 ml	F151-500
Feline Skeletal Muscle Cell Growth Medium Kit	Basal Medium & Growth Supplement packaged separately.	Yields 500ml	F151K-500
Feline Skeletal Muscle Cell Basal Medium	Add Growth Supplements before use.	500 ml	F150-500
Feline Skeletal Muscle Cell Growth Supplements		50 ml	F151-GS
Subculture Reagent Kit	(100 ml each of HBSS, Trypsin/EDTA & Neutralizer Solution)	100 ml	090K