Human Adipocytes

(HAd)

Previously, most research in adipocyte biology was conducted in mouse 3T3-L1 cell lines^1,2,3. It was impossible to use human adipocytes because they die within 24 hours after isolation. To facilitate the study of human adipocyte biology, now we provide HAd (Human Adipocytes) which were differentiated from HPAd (Human Preadipocytes) through use of Adipocyte Differentiation Medium. Cells are shipped 10 days after initiating differentiation. Mature HAd are expected 15 days after induction of differentiation and should remain healthy and responsive for at least 2 weeks after complete differentiation to mature HAd. Adipose mass can be controlled by inhibition of HPAd differentiation and increase of lypolysis. HAd are ideal cellular models for drug discovery research in the area of obesity and diabetes.

Russel, T. R. et al, Proc. Natl. Acad. Sci. USA 73:4516 (1976)
Schmidt, W. et al, J. Biol. Chem. 265:15489 (1990)
Spiegelman, B. M. et al, Cell 24:503 (1981)

Human Adipocytes, HAd

HAd

Lipid stained human adipocytes, HAd

Oil Red O stained lipid accumulation in HAd

Characterization: Positive for lipid accumulation on HAd.

Each lot is tested negative for HIV, Hepatitis B, Hepatitis C, and negative for mycoplasma, bacteria, yeast and fungi.

Product Name	Product Description	Size (vol)	Cat. No.
Proliferating HAd, adult In Flasks subcutaneous(s)	Cultured and shipped in flasks at first passage in Adipocyte Differentiation Medium.	T-25 Flask 2 T-25 Flasks T-75 Flask or 3 T-25 Flasks	803sD-25a 803sD-50a 803sD-75a
Proliferating HAd, adult In Flasks heart	Cultured and shipped in flasks at first passage in Adipocyte Differentiation Medium.	T-25 Flask 2 T-25 Flasks T-75 Flask or 3 T-25 Flasks	803hD-25a 803hD-50a 803hD-75a
Proliferating HAd, adult In Multiwell Plates subcutaneous(s)	Cultured and shipped in plates at first passage in Adipocyte Differentiation Medium.	6 well 12 well 24 well 48 well 96 well	803sD-6Wa 803sD-12Wa 803sD-24Wa 803sD-48Wa 803sD-96Wa
Proliferating HAd, adult In Multiwell Plates heart	Cultured and shipped in plates at first passage in Adipocyte Differentiation Medium.	6 well 12 well 24 well 48 well 96 well	803hD-6Wa 803hD-12Wa 803hD-24Wa 803hD-48Wa 803hD-96Wa
Adipocyte Differentiation Medium		250 ml	811D-250
Adipocyte Maintenance Medium		250 ml	811M-250
Lysis Buffer		5 ml	030-005
HAd RNA, adult subcutaneous(s)	Total RNA prepared from HAd.	10 µg 25 µg	802sD-R10a 802sD-R25a