Human Coronary Artery Endothelial Cells

(HCAEC)

Human Coronary Artery Endothelial Cells (HCAEC) are isolated from normal human coronary arteries. They are cryopreserved at second passage and can be cultured and propagated at least 15 population doublings. The endothelium are crucially involved in the regulation of coronary blood flow and cardiac functions, such as thrombosis^1,2, atherosclerosis³, and hypertension⁴. Strategically located between the circulating blood and the vascular smooth muscle, endothelial cells release numerous vasoactive substances regulating the function of vascular smooth muscle and trafficking blood cells⁵. HCAEC is an ideal candidate for the studies of endothelial cell metabolism and functional vasodilators, such as prostacyclin, bradykinin, nitric oxide and independent of the former endothelium-derived hyperpolarizing factor⁵. Co-culturing HCAEC with human coronary artery smooth muscle cells (HCASMC) could generate an in vitro model for the cell-cell interaction system³.

Jaffe, E.A., New Engl. J. Med. 296:377 (1997).
Mason, R.G. et al, Arch. Pathol. Lab. Med. 101:61 (1997).
Ross, R.N., Nature 362:801 (1993).
Ryan, U.S., Handbook of Physiology; The Respiratory System. Am. Physiol. Soc. Chap. 10:351 (1985).
Ruschitzka, F.T. et al, Cardiology, 88 Suppl 3:3-19. (1997).

human coronary artery endothelial cells, HCAEC

HCAEC

LDL uptake human coronary artery endothelial cells, HCAEC

DiI-Ac-LDL Uptake

Characterization: Positive for Factor VIII-related antigen expression. Positive for DiI-Ac-LDL uptake.

Each lot tested negative for HIV, Hepatitis B and Hepatitis C and negative for mycoplasma, bacteria, yeast, fungi.

Product Name	Product Description	Size (vol)	Cat. No.
HCAEC Total Kit, adult	Each kit contains an ampoule of cryopreserved HCAEC (300-05a), 500 ml of HCAEC Growth Medium (212-500), and a Subculture Reagent Kit (090K).	1 Kit	300K-05a
Cryopreserved HCAEC, adult	(>500,000 cells in an ampoule) Cryopreserved at second passage in Endothelial Cell Basal Medium that contains 10% FBS and 10% DMSO.	1 Ampoule	300-05a
Proliferating HCAEC, adult In Flasks	Cultured in flasks at third passage in HCAEC Growth Medium and shipped in Endothelial Cell Transfer Medium.	T-25 Flask 2 T-25 Flasks T-75 Flask or 3 T-25 Flasks	301-25a 301-50a 301-75a
Proliferating HCAEC, adult In Multiwell Plates	Cultured in plates at third passage in HCAEC Growth Medium and shipped in Endothelial Cell Transfer Medium.	6 well 12 well 24 well 48 well 96 well	301-6Wa 301-12Wa 301-24Wa 301-48Wa 301-96Wa
HCAEC Growth Medium	Optimized and ready for use	500 ml	212-500
HCAEC Growth Medium Kit	Basal Medium & Growth Supplement packaged separately	Yields 500 ml	212K-500
HCAEC Basal Medium	Add Growth Supplement before use	500 ml	210-500
HCAEC Growth Supplement		30 ml	212-GS
Subculture Reagent Kit	100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution	100 ml	090K
HCAEC RNA, adult HCAEC RNA, adult	Total RNA prepared from HCAEC Total RNA prepared from HCAEC	10 µg 25 µg	300-R10a 300-R25a