Human Epidermal Keratinocytes
(HEK)
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Human Epidermal Keratinocytes (HEK) are primary cells isolated from normal human neonatal foreskin or adult skin. They are cryopreserved at the end
of primary culture and can be cultured and propagated at least 16 population doublings. HEK is a suitable
in vitro
model for dermal toxicology tests in screening new skin products1, and for developing dermal skin substitutes2. HEK has been used in numerous applications, including
investigations of human epidermal development3, differentiation4, and cellular aspects of skin diseases5.
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Delco, V. et al, Alternative Methods in Toxicology, 3: 466 (1985).
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Joyce, S.T. et al, J. Biomed. Mater. Res. 22:937 (1988).
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Holbrook, K.A., Biochemistry and Physiology of the Skin, Vol. 1:64 (1983).
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Fuchs, F.J., Cell Biol. 111 (No. 6, Pt. 2):2807 (1990).
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Koizami, H. et al, British J. Dermatol. 134: 686 (1996).
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HEK
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HEK
stained
for
cytokeratin
peptide
14
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Characterization:
Positive for Cytokeratin Peptide
14.
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Each lot is tested negative for HIV, Hepatitis B, Hepatitis C, and negative for mycoplasma, bacteria, yeast and fungi.
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| Product Name
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Product Description
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Size (vol)
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Cat. No.
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HEK Total Kit
adult
fetal
neonatal
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Each kit contains an ampoule of cryopreserved HEK (102-05), 500 ml of HEK Cell Growth Medium (131-500) and a
Subculture Reagent Kit.
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1 Kit
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102K-05a
102K-05f
102K-05n
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Cryopreserved HEK
adult
fetal
neonatal
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(>500,000 cells in an ampoule)
Cryopreserved at the end of primary culture in Keratinocyte Basal Medium that contains 30% FBS and 10% DMSO.
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1 Ampoule
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102-05a
102-05f
102-05n
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Proliferating HEK
In Flasks
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Cultured and shipped in flasks at first passage in Keratinocyte Serum-Free Growth Medium
*Specify adult (a), fetal (f) or neonatal (n)
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T-25 Flask
2 T-25 Flasks
T-75 Flask or 3 T-25 Flasks
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103-25 *
103-50 *
103-75 *
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Proliferating HEK
In Multiwell Plates
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Cultured and shipped in plates at first passage in Keratinocyte Serum-Free Growth Medium.
*Specify adult (a), fetal (f) or neonatal (n)
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6 well
12 well
24 well
48 well
96 well
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103-6W *
103-12W *
103-24W *
103-48W *
103-96W *
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| HEK Growth Medium
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Optimized and ready for use
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500 ml
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131-500
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| HEK Growth Medium Kit
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Basal Medium & Growth Supplement packaged separately.
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Yields 500 ml
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131K-500
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| HEK Basal Medium
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Add Growth Supplement before use
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500 ml
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130-500
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| HEK Growth Supplement
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5 ml
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131-GS
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| HEK Calcium-Free Growth Medium
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500 ml
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133-500
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| HEK Calcium-Free Growth Medium Kit
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Yields 500 ml
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133K-500
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| HEK Calcium-Free Basal Medium
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500 ml
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132-500
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| HEK Calcium-Free Growth Supplement
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5 ml
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133-GS
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| Subculture Reagent Kit
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100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution
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100 ml
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090K
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HEK RNA
adult
neonatal
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Total RNA prepared from HEK
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10 µg
25 µg
10 µg
25 µg
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102-R10a
102-R25a
102-R10n
102-R25n
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