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Human
Marrow Stromal Cells (HMSC) are isolated from human bone marrow. They
are cryopreserved at second passage and can be cultured and propagated
10 population doublings.
In addition to supporting hematopoietic cells1,
marrow stromal cells can be induced to differentiate into cells of
different connective tissue lineage, such as bone, cartilage, and fat2,3,4.
The potential of HMSCs to maintain multipotency and proliferate
extensively in vitro provides new avenues for cell-based therapy
in the restoration of damaged or diseased tissue5,6. Recent
reports indicate that HMSCs are capable of cell fates crossing germ
layer boundaries. In addition to differentiating into multi-lineages of
the mesoderm, these cells can also differentiate into neurons7 of
ectodermal origin and hepatocyte-like cells8 of endodermal
origin.
1.
Verfaillie, C.M., Blood 82:1045
(1993).
2.
Bruder, S.P. et al, J. Cell.
Biochem. 64:278 (1997).
3.
Mackay, A..M. et al, Tissue
Engineering 4:415 (1998).
4.
Pittenger, M.F. et al, Science
284:143 (1999).
5.
Horwitz, E.M. et al, Blood 92
Suppl 1:249 (1998).
6.
Bruder, S.P. et al, J. Orthop.
Res. 16:155 (1998).
7.
Woodbury, D., et al, J. Neurosci.
Res. 61:364 (2000).
8.
Oh, S.H., et al, Biochem. Biophy.
Res. Commun. 279:500 (2000).
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HMSC
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von Kossa Stain
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