Human Follicle Outer Root Sheath Cells

(HORSC)

Human Follicle Outer Root Sheath Cells (HORSC) are primary cells isolated from the outer root sheath of the hair follicle and are cryopreserved at third passage. HORSC can be propagated at least 4 doublings. Outer root sheath cells can be cultured in vitro to form epidermal equivalents¹, and can be used for autologous grafting². Furthermore, HORSC are reported to support stem cells in the bulge region of the outer sheath³. These bulge cells are mutlipotent, critical for the self-renewal of hair follicles³, and also have the capacity to regenerate sebaceous glands and epidermis⁴.

1. Lenoir, MC et al., Dev Viol. 130(2):610 (1988).

2. Limat, A & Hunziker, T. Cells Tissues Organs. 172(2):79 (2002).

3. Ohyama, M et al., J Cell Invest. 116(1):19 (2006).

4. Ohyama, M, J Dermatol Sci. (2007).

Each lot tested negative for HIV, Hepatitis B and Hepatitis C and negative for mycoplasma, bacteria, yeast, fungi.

Product Name	Product Description	Size (vol)	Cat No.
HORSC Total Kit	Each kit contains an ampoule of cryopreserved HORSC (#606-05a-05), 500 ml of Outer Root Sheath Growth Medium (617-500), and a Subculture Reagent Kit (090K).	1 kit	606-05a
Cryopreserved HORSC, adult	(>500,000 cells in an ampoule) Cryopreserved in ampoules at second passage in Endothelial Cell Basal Medium that contains 10% FBS and 10% DMSO.	1 ampoule	606-05a
Proliferating HORSC, adult	Cultured in flasks at third passage in Endothelial Cell Growth Medium and shipped in Endothelial Cell Transfer Medium.	T-25 flask 2 T-25 flasks T-75 flask or 3 T-25 flasks	607-25 607-50 607-75
Proliferating HORSC, adult	Cultured in plates at third passage in Endothelial Cell Growth Medium and shipped in Endothelial Cell Transfer Medium.	6 well plate 12 well plate 24 well plate 48 well plate 96 well plate	607-6W 607-12W 607-24W 607-48W 607-96W
Outer Root Sheath Growth Medium	Optimized and ready for use.	500 ml	617-500
Outer Root Sheath Growth Medium Kit	Basal Medium & Growth Supplement packaged separately.	Yields 500ml	617K-500
Outer Root Sheath Basal Medium	Add Growth Supplements before use.	500 ml	616-500
Outer Root Sheath Growth Supplements		30 ml	617-GS
Subculture Reagent Kit	(100 ml each of HBSS, Trypsin/EDTA & Neutralizer Solution)	100 ml	090K