Human Skeletal Muscle Cells

Human Skeletal Muscle Cells

HSkMC

Human Skeletal Muscle Cells (HSkMC) are isolated from the limbal skeletal muscle. They are cryopreserved at second passage and can be cultured and propagated for at least 15 population doublings. Human Skeletal Muscle Cells can undergo differentiation to exhibit actin and myosin myofilaments. HSkMC have been used in the study of genetic muscular diseases such as Malignant Hyperthermia¹. HSkMC also have the potential to act as a cardiac graft, mending damage to the heart². Recently, cultured Human Skeletal Muscle cells have been used in micro gravity experiments to study the effects of low gravity environments on Human Skeletal Muscle³.

O’Sullivan, G.H. et al, Biochem Pharmacol 61(12):1479-1485 (2001)
El Oakley, R.M. et al, Ann. Thorac. Surg. 71(5):1724-1733 (2001)
Slentz, D.H. et al, In Vitro Cell. Dev. Biol. Anim. 37(3):148-156 (2001)

Skeletal Muscle Cells

Skeletal Muscle Cells fused to Multinucleated myotube

Characterization: Positive for sarcomere myosin.

Each lot tested negative for HIV, Hepatitis B and Hepatitis C and negative for mycoplasma, bacteria, yeast, fungi.

Learn about Cell Applications, Inc.'s Pre-Screened Skeletal Muscle Cells...

Product Name	Product Description	Size (vol)	Cat. No.
HSkMC Total Kit, , fetal	Each kit contains an ampoule of cryopreserved HSkMC (150-05f), 500 ml of Skeletal Muscle Cell Growth Medium (151-500), and a Subculture Reagent Kit.	1 Kit	150K-05f
Cryopreserved HSkMC , fetal	(>500,000 cells in an ampoule) Cryopreserved at second passage in Skeletal Muscle Cell Basal Medium that contains 10% FBS and 10% DMSO.	1 Ampoule	150-05f
Proliferating HSkMC , fetal In Flasks	Cultured in flasks at third passage in Skeletal Muscle Cell Growth Medium and shipped in Skeletal Muscle Cell Transfer Medium	T-25 Flask 2 T-25 Flasks T-75 Flask or 3 T-25 Flasks	151-25f 151-50f 151-75f
Proliferating HSkMC , fetal In Multiwell Plates	Cultured in plates at third passage in Skeletal Muscle Cell Growth Medium and shipped in Skeletal Muscle Cell Transfer Medium	6 well 12 well 24 well 48 well 96 well	151-6Wf 151-12Wf 151-24Wf 151-48Wf 151-96Wf
Skeletal Muscle Cell Growth Medium	Optimized and ready for use	500 ml	151-500
Skeletal Muscle Cell Growth Medium Kit	Basal Medium & Growth Supplement packaged separately	Yields 500 ml	151K-500
Skeletal Muscle Cell Basal Medium	Add Growth Supplement before use	500 ml	150-500
Skeletal Muscle Cell Growth Supplement		50 ml x 2	151-GS
Skeletal Muscle Cell Differentiation Medium		250 ml	151D-250
Subculture Reagent Kit	100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution	100 ml	090K
HSkMC RNA , fetal Differentiated HSkMC Total RNA , fetal	Total RNA prepared from HSkMC Multinucleated Myotubes.	10 µg 10 µg	150-R10f 150D-R10f
HSkMC RNA , fetal Differentiated HSkMC Total RNA , fetal	Total RNA prepared from HSkMC Multinucleated Myotubes.	25 µg 25 µg	150-R25f 150D-R25f