b-Galactosidase Assay Kit:
Red (CPRG)
b-Galactosidase
Assay Kit: Yellow (ONPG)
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β-Gal
Assay Kits provide tools for convenient, sensitive,
and accurate quantitation of β-Gal expression. Two
β-Galactosidase Assay Kits are available:
the highly sensitive β -Galactosidase
Assay Kit: Red (CPRG) Assay Kit, which uses the red
substrate chlorophenol red-β-D-galactopyranoside (CPRG) to determine β-gal
expression levels, and the β -Galactosidase
Assay Kit: Yellow (ONPG) Assay Kit, which measures
development of the bright yellow substrate o-nitrophenyl-β-D-galactopyranoside
(ONPG). The ONPG kit provides a quick and sensitive
method for quantifying β-galactosidase expression in
cultured cells or tissues, while the CPRG kit is
ideal for conditions in which β-galactosidse
expression levels are low.
Both β-Galactosidase Assay Kits are shipped
frozen. The CPRG substrate solutions and β-galactosidase
enzyme standard should be stored at -20șC.
All other components should be stored at 4șC.
Stable for 6 months when stored properly.
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Product
Name
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Product Description
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Size
(vol)
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Cat No.
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b-Galactosidase Assay Kit:
Red (CPRG)
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5x
Lysis Buffer (55 ml), Standard Dilution Buffer (55
ml), 10x CPRG Substrate Stock (Chlorophenol red-β-D-galactopyranoside)
(5 vials of 1 ml), Substrate Buffer (55 ml), Stop
Buffer (55 ml), β-galactosidase enzyme
standard, 40 units (100 ml)
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1
kit
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GA10-100K
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b-Galactosidase
Assay Kit:
Yellow (ONPG)
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5x
Lysis Buffer (55 ml), Standard Dilution Buffer (55
ml), ONPG Substrate Solution, 3mg/ml (o-nitrophenyl-β-D-galactopyranoside)
(55 ml), Substrate Buffer (55 ml), Stop Buffer (55
ml), β-galactosidase enzyme standard, 40 units
(100 ml)
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1
kit
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GA10-200K
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