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β-Gal
Assay Kits provide tools for rapid, convenient and
sensitive detection of β-Gal activity. The
X-Gal Staining Assay Kit is designed for sensitive
and low-background in situ visualization of
cells carrying the lacZ gene.
The β-galactosidase enzyme catalyzes the
hydrolysis of X-gal (5-bromo-3-indoyl-β-D-
galactopyranoside),
a β-galactoside. Following fixation and
incubation with the X-gal substrate, cells
transfected with a β-galactosidase-expressing
plasmid will appear blue. These blue cells can
be easily visualized by microscopy. Expression can
be detected in cell culture dishes or in tissues in
vivo. This simple and rapid assay is suitable
for expression studies in cultured cells and tissues
transfected in vivo. The
X-gal Staining Assay Kit provides all the reagents
necessary for performing 50 assays in 60 mm dishes.
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b-Gal
staining in BHK and CHO-K1 cells.
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