Oligonucleotide Transfection Reagent

Oligonucleotide Transfection Reagent

Oligonucleotide Transfection Reagent is a specially formulated lipid created to deliver oligonucleotides into a broad spectrum of cells with high efficiency and low cytotoxicity.

Antisense oligonucleotides, delivered into cells with cationic lipids, are standard tools for the elucidation of gene function. However, there are two main problems associated with lipid-mediated delivery of antisense oligonucleotides: poor transfection efficiency and/or high levels of cytotoxicity. Oligonucleotide Transfection Reagent alleviates both of these problems, delivering oligonucleotides efficiently into a broad spectrum of cell lines.

Cell Line	Species	Type
COS-7	Monkey	Kidney
CV-1	Monkey	Kidney
BalbC-3T3	Mouse	Embryo fibroblast
Rat-2	Rat	Embryo
HeLa	Human	Cervical carcinoma
NHDF	Human	Dermal fibroblast
MCF-7	Human	Breast carcinoma
A549	Human	Lung carcinoma
T24	Human	Bladder carcinoma
HCT116	Human	Colon carcinoma
H460	Human	Lung carcinoma
WiDR	Human	Colon carcinoma
HT-29	Human	Colon carcinoma
CasKi	Human	Cervical carcinoma
SiHa	Human	Cervical carcinoma

Oligonucleotide Transfection Reagent is shipped on ice. Store all reagents at 4ºC upon receipt. If stored properly, all components are stable for 3 months. For longer term stability store at -70ºC.

Transfection of antisense oligonucleotide (ASO) into HCT116, a human colon adenocarcinoma cell line. The target gene is c-raf, a well characterized Ser/Thr kinase. OTR, Oligonucleotide Transfection Reagent.

A. Dose response of c-raf in HCR116.

B. Total RNA measured with Ribogreen for corresponding treatments from Figure A.

C. Effect of Oligonucleotide Transfection Reagent alone on cell viability/toxicity.

Product Name

Product Description

Size (vol)

Cat No.

Oligonucleotide Transfection Reagent

Oligonucleotide Reagent Lipid

1 vial (1 ml)

5 vials (1 ml each)

GT610-001

GT610-005