Instructions CADMEC
Instructions HLMVEC
Instructions HAOEC
Instructions HPAEC
Instructions HUVEC
MSDS Cryopreserved Cells
5 Important Cell Culture Rules
Cell Apps Flyer Airway Cells
Cell Apps Flyer Cardiovascular Cells
Cell Apps Flyer Endothelial Cells
Cell Apps Flyer HUVEC
Cell Appls Flyer Skin Cells
Cell Apps Poster Primary Cells
Description
Human Endothelial Cells: Pre-Screened for Angiogenesis & VEGF Signaling - The most commonly used human endothelial cells are Human Umbilical Vein Endothelial Cells, (HUVEC), Human Microvascular Endothelial Cells (HMVEC) and Human Aortic Endothelial Cells (HAOEC). At Cell Applications, select lots of HUVEC, HMVEC, and HAOEC have been pre-screened to demonstrate stimulation-dependent angiogenesis and key endothelial cell signaling pathways (phosphorylation of VEGFR, Akt, MAPK, and expression of Tie2, eNOS, Axl and Etk/Bmx. Other cell types can be pre-screened by customer request.
VEGF-Stimulated Signaling in Pre-Screened Endothelial Cells -VEGF receptor-2 (VEGFR-2) is a major VEGF of endothelial cells. VEGFR-2-mediated signaling plays a critical role in angiogenesis, including regulation of proliferation, differentiation, cell movement, and survival of endothelial cells. VEGF-induced receptor dimerization triggers activation of VEGFR-2 tyrosine kinase and autophosphorylation at a specific set of tyrosine residues, which serve as docking sites for downstream signaling components leading to activation of downstream signaling molecules, including phosphorylation of Akt and p44/42-MAPK.
(Click to Enlarge) Pre-Screened Endothelial Cells Top: Activation of VEGFR-2 signaling in HAOEC, HUVEC and HMVEC by 100 ng/ml VEGF for 0, 5 or 15 minutes. VEGFR-2 autophosphorylation probed w/ phospho-VEGFR-2 (Y1175) specific Ab, Akt activation detected by phospho-Akt (S473) specific antibody, and MAP kinase activation detected using phospho-p44/42 MAP kinase (T202/Y204) Ab. Bottom: Pre-screened HUVEC & HMVEC express key endothelial markers Tie2, eNOS, Axl and Etk/Bmx.
Details
Expression of Signaling Biomarkers in Pre-Screened Endothelial Cells. Prescreened Endothelial Cells from Cell Applications, Inc. express the following important biomarkers:
- Tie2, a receptor tyrosine kinase critical for the angiogenic remodeling, sprout formation, survival of endothelial cells and vessel stabilization processes
- eNOS (endothelial Nitric Oxide Synthase), the enzyme that producse NO which is an important signaling molecule that regulates a diverse range of physiological events and is required for normal endothelial function
- Axl, a receptor tyrosine kinase whose ligand is the survival factor Gas6 (growth arrest-specific gene 6 product), is implicated in cell survival, leukocyte transmigration and neointima formation
- Etk/Bmx (Endothelial/epithelial Tyrosine Kinase), a member of the Btk family, participates in signal transduction stimulated by growth factor receptors, cytokine receptors, G-protein-coupled receptors, antigen receptors, and integrins, and has been implicated in cell adhesion, migration, proliferation and survival.
Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Cryopreserved Pre-Screened Cryopreserved Dermal Microvascular Endothelial Cell Total Kit: 5x10^5 Cells (Pre-Screened, Adult), Medium & Subculture Reagents (See Details tab for specifics) | Size: 1 Kit | CAT.#: S100K-05a | Price: $1,122.00 | |
| Cryopreserved Dermal Microvascular Endothelial Cells (CADMEC), Pre-Screened, adult: Frozen CADMEC/HMVEC (5x10^5) | Size: 1 Cryovial | CAT.#: S100-05a | Price: $825.00 | |
| Cryopreserved Pre-Screened Cryopreserved Dermal Microvascular Endothelial Cell Total Kit: 5x10^5 Cells (Pre-Screened, Neonatal), Medium & Subculture Reagents (See Details tab for specifics) | Size: 1 Kit | CAT.#: S100K-05n | Price: $1,122.00 | |
| Cryopreserved Dermal Microvascular Endothelial Cells (CADMEC), Pre-Screened, neonatal: Frozen CADMEC (5x10^5) | Size: 1 Cryovial | CAT.#: S100-05n | Price: $825.00 | |
| Cryopreserved Pre-Screened Human Aortic Endothelial Cells Total Kit: 5x10^5 Cells (Pre-Screened, Adult), Medium & Subculture Reagents (See Details tab for specifics) | Size: 1 Kit | CAT.#: S304K-05a | Price: $1,070.00 | |
| Cryopreserved Aortic Endothelial Cells (HAOEC), Pre-Screened, adult: Frozen HAOEC (5x10^5) | Size: 1 Cryovial | CAT.#: S304-05a | Price: $896.00 | |
| Cryopreserved Lung Microvascular Endothelial Cells (HLMVEC), Pre-Screened, adult: Frozen HLMVEC (5x10^5) | Size: 1 Cryovial | CAT.#: S540-05a | Price: $926.00 | |
| Cryopreserved Pre-Screened Human Lung Microvascular Endothelial Cells Total Kit: 5x10^5 Cells (Pre-Screened, Adult), Medium & Subculture Reagents (See Details tab for specifics) | Size: 1 Kit | CAT.#: S540K-05a | Price: $1,184.00 | |
| Cryopreserved Pulmonary Artery Endothelial Cells, Asthma (HPAEC-AS), Pre-Screened, adult: Frozen HPAEC-AS from donor with Asthma (5x10^5) | Size: 1 Cryovial | CAT.#: S302AS-05a | Price: $945.00 | |
| Cryopreserved Pre-Screened Human Pulmonary Artery Endothelial Cells with Asthma Total Kit: 5x10^5 Cells (from donor with Asthma, Pre-Screened, Adult), Medium & Subculture Reagents (See Details tab for specifics) | Size: 1 Kit | CAT.#: S302ASK-05a | Price: $1,119.00 | |
| Cryopreserved Pre-Screened Cryopreserved S-HUVEC Total Kit, neonatal: 5x10^5 Cells (Pre-Screened, Neonatal), Medium & Subculture Reagents (See Details tab for specifics) | Size: 1 Kit | CAT.#: S200K-05n | Price: $619.00 | |
| Cryopreserved Umbilical Vein Endothelial Cells (HUVEC), Pre-Screened, neonatal : Frozen HUVEC (5x10^5) | Size: 1 Cryovial | CAT.#: S200-05n | Price: $445.00 | |
| Proliferating Dermal Microvascular Endothelial Cells (S-CADMEC), Pre-Screened, adult: Actively growing, dividing cells in medium | Size: T-25 Flask | CAT.#: S101-25a | Price: $825.00 | |
| Proliferating Dermal Microvascular Endothelial Cells (S-CADMEC), Pre-Screened, adult: Actively growing, dividing cells in medium | Size: T-75 Flask | CAT.#: S101-75a | Price: $1,015.00 | |
| Proliferating Dermal Microvascular Endothelial Cells (S-CADMEC), Pre-Screened, adult: Actively growing, dividing cells in medium | Size: 24 Well | CAT.#: S101-24Wa | Price: $1,015.00 | |
| Proliferating Dermal Microvascular Endothelial Cells (S-CADMEC), Pre-Screened, adult: Actively growing, dividing cells in medium | Size: 96 Well | CAT.#: S101-96Wa | Price: $1,135.00 | |
| Proliferating Dermal Microvascular Endothelial Cells (S-CADMEC), Pre-Screened, neonatal : Actively growing, dividing cells in medium | Size: T-25 Flask | CAT.#: S101-25n | Price: $825.00 | |
| Proliferating Dermal Microvascular Endothelial Cells (S-CADMEC), Pre-Screened, neonatal : Actively growing, dividing cells in medium | Size: T-75 Flask | CAT.#: S101-75n | Price: $1,015.00 | |
| Proliferating Dermal Microvascular Endothelial Cells (S-CADMEC), Pre-Screened, neonatal : Actively growing, dividing cells in medium | Size: 24 Well | CAT.#: S101-24Wn | Price: $1,015.00 | |
| Proliferating Dermal Microvascular Endothelial Cells (S-CADMEC), Pre-Screened, neonatal : Actively growing, dividing cells in medium | Size: 96 Well | CAT.#: S101-96Wn | Price: $1,135.00 | |
| Proliferating Aortic Endothelial Cells (S-HAOEC), Pre-Screened, adult: Actively growing, dividing cells in medium | Size: T-25 Flask | CAT.#: S305-25a | Price: $896.00 | |
| Proliferating Aortic Endothelial Cells (S-HAOEC), Pre-Screened, adult: Actively growing, dividing cells in medium | Size: T-75 Flask | CAT.#: S305-75a | Price: $1,086.00 | |
| Proliferating Aortic Endothelial Cells (S-HAOEC), Pre-Screened, adult: Actively growing, dividing cells in medium | Size: 24 Well | CAT.#: S305-24Wa | Price: $1,086.00 | |
| Proliferating Aortic Endothelial Cells (S-HAOEC), Pre-Screened, adult: Actively growing, dividing cells in medium | Size: 96 Well | CAT.#: S305-96Wa | Price: $1,206.00 | |
| Proliferating Umbilical Vein Endothelial Cells (S-HUVEC), Pre-Screened, neonatal: Actively growing, dividing cells in medium | Size: T-25 Flask | CAT.#: S201-25n | Price: $445.00 | |
| Proliferating Umbilical Vein Endothelial Cells (S-HUVEC), Pre-Screened, neonatal: Actively growing, dividing cells in medium | Size: T-75 Flask | CAT.#: S201-75n | Price: $635.00 | |
| Proliferating Umbilical Vein Endothelial Cells (S-HUVEC), Pre-Screened, neonatal: Actively growing, dividing cells in medium | Size: 96 Well | CAT.#: S201-96Wn | Price: $755.00 |
Related Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Human EC Basal Medium: Basal medium (contains no growth supplement). Add GS before use. | Size: 500 ml | CAT.#: 210-500 | Price: $91.00 | |
| Human EC Serum-Free Defined Medium: Defined medium without serum designed to maintain endothelial cell monlayers in confluent cultures. Not for cell growth. | Size: 500 ml | CAT.#: 113-500 | Price: $130.00 | |
| Human EC Serum-Free Defined Medium wo Phenol Red: Defined medium, without serum or phenol red, designed to maintain endothelial cell monlayers in 100% confluent culture. Not intended for cell growth. | Size: 500 ml | CAT.#: 113PR-500 | Price: $136.00 | |
| Human EC Growth Medium: All-in-one ready-to-use. Optimized and best-suited for angiogenesis and other physiological studies. For accelerated growth, use Cat# 213-500. | Size: 500 ml | CAT.#: 211-500 | Price: $121.00 | |
| Human Meso Endo Growth Medium wo Antibiotics: Growth medium without antibiotics | Size: 500 ml | CAT.#: 212A-500 | Price: $143.00 | |
| Human EC Growth Medium Kit: Basal medium & growth supplement sold together packaged separately. Optimized and best-suited for angiogenesis and other physiological studies. For accelerated growth, use Cat# 213-500. | Size: Yields 500 ml | CAT.#: 211K-500 | Price: $143.00 | |
| Human EC Growth Supplement: Added to Basal Medium to create Growth Medium. The complete Growth Medium (equivalent to Cat# 211-500) is optimized and best-suited for angiogenesis and other physiological studies. For accelerated growth, use Cat# 213-500. | Size: 15 ml | CAT.#: 211-GS | Price: $74.00 | |
| Human EC Growth Supplement wo Antibiotics: Growth supplement without antibiotics | Size: 15 ml | CAT.#: 211A-GS | Price: $88.00 | |
| Human EC Starvation Medium: Use when cells need to be starved overnight to 24 hrs before experiment | Size: 250 ml | CAT.#: 209-250 | Price: $81.00 | |
| Human EC Starvation Medium wo Phenol Red: Starvation medium without phenol red | Size: 250 ml | CAT.#: 209PR-250 | Price: $91.00 | |
| Human Meso Endo Growth Medium: All-in-one ready-to-use | Size: 500 ml | CAT.#: 212-500 | Price: $136.00 | |
| Human Meso Endo Growth Medium wo Phenol Red: Growth medium without phenol red | Size: 500 ml | CAT.#: 212PR-500 | Price: $136.00 | |
| Human Meso Endo Growth Medium Kit: Basal medium & growth supplement sold together packaged separately | Size: Yields 500ml | CAT.#: 212K-500 | Price: $147.00 | |
| Human Meso Endo Growth Medium Kit wo Phenol Red: Growth medium kit without phenol red | Size: yields 500 ml | CAT.#: 212KPR-500 | Price: $150.00 | |
| Human Meso Endo Growth Supplement: Added to Basal Medium to create Growth Medium | Size: 30 ml | CAT.#: 212-GS | Price: $81.00 | |
| Human Meso Endo Growth Medium wo FBS: Growth Medium without FBS | Size: 500 ml | CAT.#: 212F-500 | Price: $156.00 | |
| Human Meso Endo Growth Supplement wo FBS: Growth Supplement without FBS | Size: 5 ml | CAT.#: 212F-GS | Price: $88.00 |
Extended Family Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Anti-ICAM-1: Rabbit Intercellular Adhesion Molecule-1 Antibody | Size: 100 ul | CAT.#: CG1238 | Price: $275.00 | |
| Polyclonal Vascular Endothelial Growth Factor-C Antibody: Polyclonal Vascular Endothelial Growth Factor-C Antibody | Size: 100 ul | CAT.#: CB3778 | Price: $302.00 | |
| Polyclonal VEGF Receptor 1 Antibody: Polyclonal VEGF Receptor 1 Antibody | Size: 100 ul | CAT.#: CB3839 | Price: $333.00 | |
| Dermal Microvascular Endothelial Cell RNA (CADMEC RNA), Adult: Total RNA prepared from Human Dermal Microvascular Endothelial Cells, adult | Size: 10 ug | CAT.#: 100-R10a | Price: $398.00 | |
| Dermal Microvascular Endothelial Cell RNA (CADMEC RNA), Neonatal: Total RNA prepared from Human Dermal Microvscular Endothelial Cells, neonatal | Size: 10 ug | CAT.#: 100-R10n | Price: $398.00 | |
| Dermal Microvascular Endothelial Cell RNA (CADMEC RNA), Adult: Total RNA prepared from Human Dermal Microvascular Endothelial Cells, adult | Size: 25 ug | CAT.#: 100-R25a | Price: $796.00 | |
| Dermal Microvascular Endothelial Cell RNA (CADMEC RNA), Neonatal: Total RNA prepared from Human Dermal Microvscular Endothelial Cells, neonatal | Size: 25 ug | CAT.#: 100-R25n | Price: $796.00 | |
| Freezing Medium: For general cryopreservation of most primary cells. Contains FBS & DMSO. | Size: 50 ml | CAT.#: 040-50 | Price: $54.00 | |
| Cyto-X Cell Counting Reagent: 500 tests | Size: 1 Bottle | CAT.#: 028-01 | Price: $160.00 | |
| Cyto-X Cell Counting Reagent Sample: 100 tests | Size: Sample | CAT.#: 028-S | Price: $41.00 | |
| Cytofect Endothelial Cell Transfection Kit (250 x 24-Wells): 250 x 24-Well Rxns | Size: 1 Kit | CAT.#: TF101K | Price: $496.00 | |
| Cytofect Endothelial Cell Transfection Sample Kit (25 x 24-Wells): 25 x 24-Well Rxns | Size: 1 Sample Kit | CAT.#: TF101KS | Price: $62.00 | |
| Human E-Selectin ELISA Kit: Human E-Selectin ELISA Kit | Size: 96 Wells | CAT.#: CL0501 | Price: $587.00 | |
| Aortic Endothelial Cell RNA (HAOEC RNA), Adult: Total RNA prepared from Human Aortic Endothelial Cells, adult | Size: 10 ug | CAT.#: 304-R10a | Price: $398.00 | |
| Aortic Endothelial Cell RNA (HAOEC RNA), Adult: Total RNA prepared from Human Aortic Endothelial Cells, adult | Size: 25 ug | CAT.#: 304-R25a | Price: $796.00 | |
| Pulmonary Artery Endothelial Cell RNA (HPAEC RNA), Adult: Total RNA prepared from Human Pulmonary Artery Endothelial Cells, adult | Size: 10ug | CAT.#: 302-R10a | Price: $398.00 | |
| Pulmonary Artery Endothelial Cell RNA (HPAEC RNA), Adult: Total RNA prepared from Human Pulmonary Artery Endothelial Cells, adult | Size: 25ug | CAT.#: 302-R25a | Price: $796.00 | |
| Umbilical Vein Endothelial Cell RNA (HUVEC RNA), Neonatal: Total RNA prepared from Human Umbilical Vein Endothelial Cells, neonatal | Size: 10 ug | CAT.#: 200-R10n | Price: $398.00 | |
| Umbilical Vein Endothelial Cell RNA (HUVEC RNA), Neonatal: Total RNA prepared from Human Umbilical Vein Endothelial Cells, neonatal | Size: 25 ug | CAT.#: 200-R25n | Price: $796.00 | |
| Human ICAM-1 ELISA Kit: Human Intercellular Adhesion Molecule-1 ELISA Kit | Size: 96 Wells | CAT.#: CL0370 | Price: $484.00 | |
| Human Gamma-Interferon Inducible Protein 10 (IP-10 / CXCL10): Human gamma-Interferon Inducible Protein 10 | Size: 25 ug | CAT.#: RP1127-25 | Price: $194.00 | |
| Human Gamma-Interferon Inducible Protein 10 (IP-10 / CXCL10): Human gamma-Interferon Inducible Protein 10 | Size: 100 ug | CAT.#: RP1127-100 | Price: $484.00 | |
| Human Gamma-Interferon Inducible Protein 10 (IP-10 / CXCL10): Human gamma-Interferon Inducible Protein 10 | Size: 1000 ug | CAT.#: RP1127-1000 | Price: $3,175.00 | |
| Human P-Selectin ELISA Kit: Human P-Selectin ELISA Kit | Size: 96 wells | CAT.#: CL0505 | Price: $517.00 | |
| Subculture Reagent Kit: 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution | Size: 1 Kit | CAT.#: 090K | Price: $63.00 | |
| Human Vascular Endothelial Growth Factor-121 (VEGF-121): Human Vascular Endothelial Growth Factor-121 | Size: 10 ug | CAT.#: RP1116-10 | Price: $194.00 | |
| Human Vascular Endothelial Growth Factor-121 (VEGF-121): Human Vascular Endothelial Growth Factor-121 | Size: 100 ug | CAT.#: RP1116-100 | Price: $484.00 | |
| Human Vascular Endothelial Growth Factor-121 (VEGF-121): Human Vascular Endothelial Growth Factor-121 | Size: 1000 ug | CAT.#: RP1116-1000 | Price: $4,090.00 | |
| Human VEGF-c ELISA Kit: Human Vascular Endothelial Growth Factor C ELISA Kit | Size: 96 Wells | CAT.#: CL0588 | Price: $581.00 | |
| Human VEGF-121, Animal-Free: Human Vascular Endothelial Growth Factor-121, Animal-Free | Size: 10 ug | CAT.#: RP1116AF-10 | Price: $213.00 | |
| Human VEGF-121, Animal-Free: Human Vascular Endothelial Growth Factor-121, Animal-Free | Size: 100 ug | CAT.#: RP1116AF-100 | Price: $533.00 | |
| Human VEGF-121, Animal-Free: Human Vascular Endothelial Growth Factor-121, Animal-Free | Size: 1000 ug | CAT.#: RP1116AF-1000 | Price: $4,499.00 |
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