MSDS Cryopreserved Cells
Instructions BCAEC
5 Important Cell Culture Rules
Cell Apps Fllyer Cardiovascular Cells
Cell Apps Flyer Endothelial Cells
Cell Apps Poster Primary Cells
Cell Applications Inc Brochure
Description
Bovine Coronary Artery Endothelial Cells (BCAEC) from CAI have been used to demonstrate that stress hormone cortisol inhibits NO synthase and Ca2+ mobilization, leading to decreased NO release by endothelial cells, resulting in increased arterial contraction and decreased blood flow. A Similar action mechanism was determined for cocaine effects on arteries. Additionally, it was found that cocaine-induced apoptosis of endothelial cells is mediated by attenuation of NO production, down-regulation of Bcl-2 and translocation of Bac to the mitochondria, followed by release of cytochrome c and activation of caspase 9 and caspase 3. BCAEC were also used to determine diabetes-related vascular dysfunction. Others employed the cells to evaluate novel mesh-like materials for their suitability for biomedical applications.
(Click to Enlarge) Bovine Coronary Artery Endothelial Cells: BCAEC. Isolated from blood vessel tissue.
Details
Tissue | Normal healthy bovine coronary artery |
QC | No bacteria, yeast, fungi, mycoplasma |
Character | DiI-Ac-LDL uptake: Positive |
Bioassay | Attach, spread, proliferate in Growth Med |
Cryovial | 500,000 BCAEC (2nd passage) frozen in Basal Medium w/ 10% FBS & 10% DMSO |
Kit | Cryovial frozen BCAEC (B300-05), Growth Med (B211-500), Subcltr Rgnt Kit (090K) |
Proliferating | Shipped in Gr Med, 4th psg (flasks or plates) |
Doublings | At least 16 |
Applications | Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use. |
Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Cryopreserved Bovine Coronary Artery Endothelial Cells Total Kit: 5x10^5 Cells, Medium & Subculture Reagents (See Details tab for specifics) | Size: 1 Kit | CAT.#: B300K-05 | Price: $869.00 | |
| Cryopreserved Bovine Coronary Artery Endothelial Cells (BCAEC): Frozen BCAEC (5x10^5) | Size: 1 Cryovial | CAT.#: B300-05 | Price: $695.00 | |
| Proliferating Bovine Coronary Artery Endothelial Cells (BCAEC): Actively growing, dividing cells in medium | Size: T-25 Flask | CAT.#: B301-25 | Price: $695.00 | |
| Proliferating Bovine Coronary Artery Endothelial Cells (BCAEC): Actively growing, dividing cells in medium | Size: T-75 Flask | CAT.#: B301-75 | Price: $885.00 | |
| Proliferating Bovine Coronary Artery Endothelial Cells (BCAEC): Actively growing, dividing cells in medium | Size: 24 Well | CAT.#: B301-24W | Price: $885.00 | |
| Proliferating Bovine Coronary Artery Endothelial Cells (BCAEC): Actively growing, dividing cells in medium | Size: 96 Well | CAT.#: B301-96W | Price: $1,005.00 |
Related Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Bovine EC Basal Medium : Basal medium (contains no growth supplement). Add GS before use. | Size: 500 ml | CAT.#: B210-500 | Price: $74.00 | |
| Bovine EC Growth Medium: All-in-one ready-to-use | Size: 500 ml | CAT.#: B211-500 | Price: $121.00 | |
| Bovine EC Growth Medium Kit: Basal medium & growth supplement sold together packaged separately | Size: Yields 500 ml | CAT.#: B211K-500 | Price: $130.00 | |
| Bovine EC Growth Supplement: Added to Basal Medium to create Growth Medium | Size: 30 ml | CAT.#: B211-GS | Price: $66.00 |
Extended Family Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Freezing Medium: For general cryopreservation of most primary cells. Contains FBS & DMSO. | Size: 50 ml | CAT.#: 040-50 | Price: $54.00 | |
| Cytofect Endothelial Cell Transfection Kit (250 x 24-Wells): 250 x 24-Well Rxns | Size: 1 Kit | CAT.#: TF101K | Price: $496.00 | |
| Cytofect Endothelial Cell Transfection Sample Kit (25 x 24-Wells): 25 x 24-Well Rxns | Size: 1 Sample Kit | CAT.#: TF101KS | Price: $62.00 | |
| Subculture Reagent Kit: 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution | Size: 1 Kit | CAT.#: 090K | Price: $63.00 |
Resources/Documents
Citations
Powered by BiozPublications
2017
Beltrán-Partida, E., B. Valdéz-Salas, A. Moreno-Ulloa, A. Escamilla, M. Curiel, R. Rosales-Ibáñez, F. Villarreal, D. Bastidas and J. Bastidas. 2017. Improved in vitro angiogenic behavior on anodized titanium dioxide nanotubes. J Nanobiotech, 15:10.
Hara, T., T. Kojima, H. Matsuzaki, T. Nakamura, E., Yoshida, Y. Fujiwara, C. Yamomoto, S. Saito and T. Kaji. 2017. Induction of Syndecan-4 by Organic–Inorganic Hybrid Molecules with a 1,10-Phenanthroline Structure in Cultured Vascular Endothelial Cells. Int J Mol Sci, 18:352.
2015
Moreno-Ulload, A., D. Mendez-Luna, E. Beltran-Partida, C. Castillo, G. Guevara, I. Ramirez-Sanchez, J. Correa-Basurto, G. Ceballos, and F. Villarreal. 2015. The effects of (−)-epicatechin on endothelial cells involve the G protein-coupled estrogen receptor (GPER). Pharmacological Research, doi:10.1016/j.phrs.2015.08.014.
Takizawa, Y., R. Nakata, K. Fukuhara, H. Yamashita, H. Kubodera and H. Inoue. 2015. The 4-Hydroxyl Group of Resveratrol Is Functionally Important for Direct Activation of PPARa. PLoS ONE 10(3): e0120865.
Varela, C., E. Fromentin, M. Roller, F. Villarreal, and I. Ramirez-Sanchez. 2015. Effects of a Natural Extract of Aronia melanocarpa Berry on Endothelial Cell Nitric Oxide Production. J Food Biochem, doi: 10.1111/jfbc.12226.
2008
Romero, M.J., D.H. Platt, H.E. Tawfik, M. Labazi, A.B. El-Remessy, M. Bartoli, R.B. Caldwell, and R.W. Caldwell. 2008. Diabetes-induced Coronary Vascular Dysfunction Involves Increased Arginase Activity. Circulation research. 102:95-102.
2007
Niwa, K., J. Sakai, T. Watanabe, T. Ohyama, and T. Karino. 2007. Improved arterial wall model by coculturing vascular endothelial and smooth muscle cells. In vitro cellular & developmental biology. Animal. 43:17-20.
2005
He, J., S. Yang, and L. Zhang. 2005. Effects of Cocaine on Nitric Oxide Production in Bovine Coronary Artery Endothelial Cells. Journal of Pharmacology and Experimental Therapeutics. 314:980-986.
2004
Nair, L.S., S. Bhattacharyya, J.D. Bender, Y.E. Greish, P.W. Brown, H.R. Allcock, and C.T. Laurencin. 2004. Fabrication and Optimization of Methylphenoxy Substituted Polyphosphazene Nanofibers for Biomedical Applications. Biomacromolecules. 5:2212-2220.
2002
Rogers, K.M., C.A. Bonar, J.L. Estrella, and S. Yang. 2002. Inhibitory effect of glucocorticoid on coronary artery endothelial function. American Journal of Physiology - Heart and Circulatory Physiology. 283:H1922-H1928.
2001
He, J., Y. Xiao, and L. Zhang. 2001. Cocaine-Mediated Apoptosis in Bovine Coronary Artery Endothelial Cells: Role of Nitric Oxide. Journal of Pharmacology and Experimental Therapeutics. 298:180-187.
2000
He, J., Y. Xiao, C.A. Casiano, and L. Zhang. 2000. Role of Mitochondrial Cytochrome c in Cocaine-Induced Apoptosis in Coronary Artery Endothelial Cells. Journal of Pharmacology and Experimental Therapeutics. 295:896-903.