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Instructions 072K Chondrocyte Redifferentiation Kit-Alginate
Description
Human Chondrocytes Re-Differentiated (HC-RD) have been encapsulated in an alginate bead suspension. HC-RD exhibit spherical morphology and have regained their ability to produce the phenotypic markers Aggrecan and Type II Collagen. RD-HC can be used in tissue engineering experiments aimed to repair cartilage defects and to study chondrocyte differentiation and the effects of Growth factors & Cytokines on the suppression and maintenance of differentiated cartilage phenotype.
Instructions to release HC-RD from Alginate Beads
- Remove Chondrocyte Differentiation Medium
- Wash the beads 2 times with 0.155 M NaCl
- Add 3 volume of Depolymerization Solution per volume of packed alginate beads.
- Mix at room temperature for 30 minutes with gentle agitation until the beads have completely dissolved
- Pellet re-differentiated chondrocytes at 1000 rpm for 10 minutes
- Wash the cells 2 times with 0.155 M NaCl
Media Salts & Chemicals used to Release HC-RD from alginate beads:
- Calcium Chloride Solution: Formulated to induce polymerization of Sodium Alginate.
- Sodium Alginate: Encapsulates chondrocytes to maintain their phenotypic characterization after isolation or to induce cultured chondrocytes to re-differentiate to chondrocytes
- Depolymerization Solution (DS): Consists of sodium citrate, and dissolves alginate beads to release re-differentiated chondrocytes.
(Click to Enlarge) Re-Differentiated Human Chondrocytes (HC-RD), encapsulated in an alginate bead suspension.
Details
Tissue | Normal healthy human articular cartilage |
QC | No bacteria, yeast, fungi, mycoplasma, virus |
Cryovial | N/A |
Kit | N/A |
Proliferating | Shipped in Differentiation Medium (flasks) |
Doublings | N/A |
Note | Cells shipped 10 days after encapsulation. Re-Differentiated Chondrocytes expected 3 weeks after encapsulation, retain phenotype at least 1 week after complete re-differentiation. |
Applications | Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use. |
Chondrocytes cultured in monolayer quickly lose their phenotypic characteristics and no longer express Collagen type II and sulfated proteoglycan. Encapsulation of de-differentiated chondrocytes for 3 to 4 weeks in alginate beads will induce re-differentiation and ECM production in chondrocytes.
- Calcium Chloride Solution is formulated to induce polymerization of Sodium Alginate.
- Chondrogenesis Kit contains all media & solutions for the encapsulization of chondrocytes in alginate beads
- Sodium Alginate encapsulates chondrocytes to maintain their phenotypic characterization after isolation or to induce cultured chondrocytes to re-differentiate to chondrocytes.
- Depolymerization Solution (DS) consists of sodium citrate, and dissolves alginate beads to release re-differentiated chondrocytes.
Instructions for Releasing Re-differentiated Chondrocytes from Alginate Beads
- Remove Chondrocyte Differentiation Medium
- Wash the beads 2 times with 0.155 M NaCl
- Add 3 volume of Depolymerizatiojn Solution per volume of packed alginate beads
- Mix at room temperature for 30 minutes
- Apply gentle agitation until the beads have completely dissolved
- Pellet re-differentiated chondrocytes at 1000 rpm for 10 minutes
- Wash the cells 2 times with 0.155 M NaCl
Chondrogenesis Kit (Cat. No. 072K)
| Kit Component | Cat. No. | Storage |
| Sodium Alginate Solution | 072-25 | 25C |
| Sodium Chloride Solution | 074-100 | 25C |
| Calcium Chloride Solution | 071-100 | 25C |
| Depolymerization Solution | 073-50 | 25C |
| Chondrocyte Differentiation Medium | 411D-250 | 4C |
Antibiotic Antimycotic Solution: AAS
Cat. No. 010-100 / 100 ml
10,000 units/ml Penicillin-G, 10,000 ug/ml Streptomycin Sulfate, and 25 ug/ml Amphotericin in normal saline Sterile filtered solution, 100X, dilute before use
Calcium Chloride Solution: CaCl2
Cat. No. 071-100 / 100 ml
Sterile, ready-to-use
Depolymerization Solution: DS
Cat. No. 073-50 / 50 ml
Sterile-filtered sodium citrate solution, ready-to-use
Hank’s Balanced Salt Solution: HBSS
Cat. No. 062-100 / 100 ml
Sterile 1X solution, ready-to-use
Sodium Alginate Solution
Cat. No. 072-25 / 25 ml
Sterile 1.2% solution, ready-to-use
Sodium Chloride Solution: NaCl
Cat. No. 074-100 / 100 ml
Sterile filtered 0.155 M saline solution, ready-to-use
Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Proliferating Chondrocytes, Re-Differentiated (HC-RD), adult: Actively growing, dividing cells, in medium | Size: T-25 Flask | CAT.#: 403RD-25a | Price: $909.00 | |
| Proliferating Chondrocytes, Re-Differentiated (HC-RD), adult: Actively growing, dividing cells, in medium | Size: T-75 Flask | CAT.#: 403RD-75a | Price: $1,114.00 | |
| Proliferating Chondrocytes, Re-Differentiated (HC-RD), fetal: Actively growing, dividing cells, in medium | Size: T-25 Flask | CAT.#: 403RD-25f | Price: $866.00 | |
| Proliferating Chondrocytes, Re-Differentiated (HC-RD), fetal: Actively growing, dividing cells, in medium | Size: T-75 Flask | CAT.#: 403RD-75f | Price: $1,071.00 |
Related Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| HC Basal Medium: Basal medium (contains no growth supplement). Add GS before use. | Size: 500 ml | CAT.#: 410-500 | Price: $70.00 | |
| HC Differentiation Medium: Promotes cells to change from one type to another, more specialized | Size: 250 ml | CAT.#: 411D-250 | Price: $110.00 | |
| HC Growth Medium: All-in-one ready-to-use | Size: 500 ml | CAT.#: 411-500 | Price: $140.00 | |
| HC Growth Medium wo Phenol Red: Growth medium without phenol red | Size: 500 ml | CAT.#: 411PR-500 | Price: $136.00 | |
| HC Growth Medium Kit: Basal medium & growth supplement sold together packaged separately | Size: Yields 500 ml | CAT.#: 411K-500 | Price: $128.00 | |
| HC Growth Supplement: Added to Basal Medium to create Growth Medium | Size: 50 ml | CAT.#: 411-GS | Price: $74.00 | |
| HC Differentiation Medium wo Phenol Red: Promotes cells to change from one type to another, more specialized, without Phenol Red | Size: 250 ml | CAT.#: 411DPR-250 | Price: $128.00 |
Extended Family Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Mouse MMP-1 Antibody: Mouse MMP-1 Antibody | Size: 100 ul | CAT.#: CP10422 | Price: $302.00 | |
| Freezing Medium: For general cryopreservation of most primary cells. Contains FBS & DMSO. | Size: 50 ml | CAT.#: 040-50 | Price: $54.00 | |
| Chondrogenesis Kit: Used to re-differentiate chondrocytes, that have been grown in monolayers, by encapsulation in alginate beads. | Size: 1 Kit | CAT.#: 072K | Price: $229.00 | |
| Human Connective Tissue Growth Factor (CTGF): Human Connective Tissue Growth Factor | Size: 20 ug | CAT.#: RP1060-20 | Price: $194.00 | |
| Human Connective Tissue Growth Factor (CTGF): Human Connective Tissue Growth Factor | Size: 100 ug | CAT.#: RP1060-100 | Price: $484.00 | |
| Human Connective Tissue Growth Factor (CTGF): Human Connective Tissue Growth Factor | Size: 1000 ug | CAT.#: RP1060-1000 | Price: $4,090.00 | |
| Cyto-X Cell Counting Reagent: 500 tests | Size: 1 Bottle | CAT.#: 028-01 | Price: $160.00 | |
| Cyto-X Cell Counting Reagent Sample: 100 tests | Size: Sample | CAT.#: 028-S | Price: $41.00 | |
| Cytofect Chondrocyte Transfection Kit (175 x 24-Wells): 175 x 24-Well Rxns | Size: 1 Kit | CAT.#: TF402K | Price: $496.00 | |
| Cytofect Chondrocyte Transfection Sample Kit (25 x 24-Wells): 25 x 24-Well Rxns | Size: 1 Sample Kit | CAT.#: TF402KS | Price: $62.00 | |
| Chondrocyte RNA (HC RNA), Re-Differentiated, Adult: Total RNA prepared from Human Chondrocytes, Re-Differentiated, adult | Size: 10 ul | CAT.#: 402RD-R10a | Price: $514.00 | |
| Chondrocyte RNA (HC RNA), Re-Differentiated, Adult: Total RNA prepared from Human Chondrocytes, Re-Differentiated, adult | Size: 25 ul | CAT.#: 402RD-R25a | Price: $1,028.00 | |
| Chondrocyte RNA (HC RNA), Re-Differentiated, Fetal: Total RNA prepared from Human Chondrocytes, Re-Differentiated, fetal | Size: 10 ul | CAT.#: 402RD-R10f | Price: $514.00 | |
| Chondrocyte RNA (HC RNA), Re-Differentiated, Fetal: Total RNA prepared from Human Chondrocytes, Re-Differentiated, fetal | Size: 25 ul | CAT.#: 402RD-R25f | Price: $1,028.00 | |
| Human MMP-1 ELISA Kit: Human Matrix Metalloproteinase-1 ELISA Kit | Size: 96 Wells | CAT.#: CL0458 | Price: $495.00 | |
| Subculture Reagent Kit: 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution | Size: 1 Kit | CAT.#: 090K | Price: $63.00 |