MSDS Cryopreserved Cells
Instructions HITASMC
Cell Apps Flyer Smooth Muscle Cells
Cell Apps Flyer Cardiovascular Cells
5 Important Cell Culture Rules
Cell Apps Poster Primary Cells
Description
Human Internal Thoracic Smooth Muscle Cells (HITASMC) are derived from the normal internal thoracic arteries (formerly called internal mammary arteries).
HITASMC, along with Human Coronary Artery Smooth Muscle Cells, both from Cell Applications, Inc. have been used to investigate the gene expression differences between smooth muscle cells from different arteries, underlying their differential response to injuries and proliferation stimuli.
Characterization: positive for smooth muscle cell specific alpha-actin expression
(Click to Enlarge) Human Internal Thoracic / Mammary Artery Smooth Muscle Cells: HITASMC. Cells isolated from the vessel that supplies the anterior chest wall and breasts.
Details
Tissue | Normal healthy human intrathoratic artery |
QC | No bacteria, yeast, fungi, mycoplasma, virus |
Character | Smooth muscle specific α-actin positive |
Bioassay | Attach, spread, proliferate in Growth Med |
Cryovial | 500,000 HITASMC (2nd passage) frozen in Basal Medium w/ 10% FBS, 10% DMSO |
Kit | Cryovial frozen HITASMC (358-05a), Growth Medium, (311-500), Subcltr Rgnt Kit (090K) |
Proliferating | Shipped in Gr Med, 3rd psg (flasks or plates) |
Doublings | At least 8 |
Applications | Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use. |
Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Cryopreserved Human Internal Thoracic Artery Smooth Muscle Cells Total Kit, adult: 5x10^5 Cells (Adult), Medium & Subculture Reagents (See Details tab for specifics) | Size: 1 Kit | CAT.#: 358K-05a | Price: $999.00 | |
| Cryopreserved Internal Thoracic Artery Smooth Muscle Cells (HITASMC), adult: Frozen HITASMC (5x10^5) | Size: 1 Cryovial | CAT.#: 358-05a | Price: $825.00 | |
| Proliferating Internal Thoracic Artery Smooth Muscle Cells (HITASMC), adult: Actively growing, dividing cells in medium | Size: T-25 Flask | CAT.#: 359-25a | Price: $825.00 | |
| Proliferating Internal Thoracic Artery Smooth Muscle Cells (HITASMC), adult: Actively growing, dividing cells in medium | Size: T-75 Flask | CAT.#: 359-75a | Price: $1,015.00 | |
| Proliferating Internal Thoracic Artery Smooth Muscle Cells (HITASMC), adult: Actively growing, dividing cells in medium | Size: 96 Well | CAT.#: 359-96Wa | Price: $1,135.00 |
Related Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Human SMC Basal Medium: Basal medium (contains no growth supplement). Add GS before use. | Size: 500 ml | CAT.#: 310-500 | Price: $74.00 | |
| Human SMC Differentiation Medium: Promotes cells to change from one type to another, more specialized | Size: 250 ml | CAT.#: 311D-250 | Price: $84.00 | |
| Human SMC Growth Medium: All-in-one ready-to-use | Size: 500 ml | CAT.#: 311-500 | Price: $121.00 | |
| Human SMC Growth Medium Kit: Basal medium & growth supplement sold together packaged separately | Size: Yields 500ml | CAT.#: 311K-500 | Price: $130.00 | |
| Human SMC Growth Supplement: Added to Basal Medium to create Growth Medium | Size: 30 ml | CAT.#: 311-GS | Price: $74.00 | |
| Subculture Reagent Kit: 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution | Size: 1 Kit | CAT.#: 090K | Price: $63.00 |
Extended Family Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Mouse Interleukin-6 Antibody: Mouse Interleukin-6 Antibody | Size: 100 ul | CAT.#: CP10325 | Price: $302.00 | |
| Cytofect Smooth Muscle Cell Transfection Kit (125 x 24-Wells): 125 x 24-Well | Size: 1 Kit | CAT.#: TF350K | Price: $328.00 | |
| Cytofect Smooth Muscle Cell Transfection Sample Kit (25 x 24-Wells): 25 x 24-Well Rxns | Size: 1 Sample Kit | CAT.#: TF350KS | Price: $62.00 | |
| Cyto-X Cell Counting Reagent: 500 tests | Size: 1 Bottle | CAT.#: 028-01 | Price: $160.00 | |
| Cyto-X Cell Counting Reagent Sample: 100 tests | Size: Sample | CAT.#: 028-S | Price: $41.00 | |
| Internal Thoracic Artery Smooth Muscle Cell RNA (HITASMC RNA), Adult: Total RNA prepared from Human Internal Thoracic Artery Smooth Muscle Cells, adult | Size: 10 ug | CAT.#: 358-R10a | Price: $398.00 | |
| Internal Thoracic Artery Smooth Muscle Cell RNA (HITASMC RNA), Adult: Total RNA prepared from Human Internal Thoracic Artery Smooth Muscle Cells, adult | Size: 25 ug | CAT.#: 358-R25a | Price: $796.00 | |
| Human Heart RNA: Total RNA prepared from human heart tissue | Size: 50 ug | CAT.#: 1H30-50 | Price: $158.00 | |
| Human Heart RNA: Total RNA prepared from human heart tissue | Size: 250 ug | CAT.#: 1H30-250 | Price: $595.00 | |
| Human Interleukin-6 (IL-6): Human Interleukin-6 | Size: 20 ug | CAT.#: RP1010-20 | Price: $194.00 | |
| Human Interleukin-6 (IL-6): Human Interleukin-6 | Size: 100 ug | CAT.#: RP1010-100 | Price: $484.00 | |
| Human Interleukin-6 (IL-6): Human Interleukin-6 | Size: 1000 ug | CAT.#: RP1010-1000 | Price: $3,175.00 | |
| Human IL-6 ELISA Kit: Human Interleukin-6 ELISA Kit | Size: 96 Wells | CAT.#: CL0410 | Price: $517.00 | |
| Human IL-6, Animal Free: Human Interleukin-6, Animal-Free | Size: 20 ug | CAT.#: RP1010AF-20 | Price: $213.00 | |
| Human IL-6, Animal Free: Human Interleukin-6, Animal-Free | Size: 100 ug | CAT.#: RP1010AF-100 | Price: $533.00 | |
| Human IL-6, Animal Free: Human Interleukin-6, Animal-Free | Size: 1000 ug | CAT.#: RP1010AF-1000 | Price: $3,492.00 |
Resources/Documents
Citations
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2014
Lange, M., T. Fujikawa, A. Koulova, S. Kang, M. Griffin, A. Lassaletta, A. Erat., E. Tobiash, C. Bianchi, N. Elmadhun, F. Selke, and A. Usheva. 2014. Arterial territory-specific phosphorylated retinoblastoma protein species and CDK2 promote differences in the vascular smooth muscle cell response to mitogens. Cell Cylcle, 13:315-323.
2013
Lange, M. 2013. Artery Specific Differences in Cell Cycle Regulation are Associated with Serum Induced Proliferation of Vascular Smooth Muscle Cells. MSc dissertation, University of Applied Science Bonn-Rhine-Sieg.
2011
Seymour, K., B. Sadowitz, J. Stein, J. Lawler, K. Maier, and V. Gahton. 2011. Vascular smooth muscle cell migration induced by domains of thrombospondin-1 is differentially regulated. Am J Surg, 202:553-557.