Instructions HPBB
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MSDS Cryopreserved Cells
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Description
Human Peripheral Blood B Cells (HPBB) provide a useful tool for studying various aspects of pathology and biology of human B lymphocytes in vitro. We isolate HPBB from the blood of healthy donors and cryopreserve them immediately after isolation. These are the antibody forming cells of the immune system and originate from pluripotent hematopoetic stem cells and differentiate in the fetal liver. In adults, B cells principally originate in the bone marrow. Newly generated, surface Ig-expressing mature B cells leave the bone marrow and circulate into peripheral lymphoid organs.
(Click to Enlarge) Human Peripheral Blood CD19+ B Cells: HPBB. Antibody-producing lymphocytes.
Details
Tissue | Normal healthy human peripheral blood |
| QC | No bacteria, yeast, fungi, mycoplasma or virus |
| Character | CD19 positive selection |
Cryovial | 2,000,000 HPBB frozen right after isolation in DMEM w/ 45% FBS, 10% DMSO |
Kit | Cryovial Frozen HPBB (6904-20a), Maintenance Medium (615-250) |
| Doublings | N/A - Blood cells don't proliferate in vitro |
| Applications | Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use. |
Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Cryopreserved Human Peripheral Blood CD19+ B Cells Total Kit, adult: 2x10^6 CD19+ B Cells (Adult) & Medium (See Details tab for specifics) | Size: 1 Kit | CAT.#: 6904K-20a | Price: $991.00 | |
| Cryopreserved Peripheral Blood B Cells (HPBB), adult: Frozen HPBB, CD19+ (2x10^6) | Size: 1 Cryovial | CAT.#: 6904-20a | Price: $925.00 |
Related Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Human Blood Cell Culture Medium: Optimized and ready for use. | Size: 250 ml | CAT.#: 615-250 | Price: $76.00 | |
| Human Blood Cell Culture Medium Kit: Basal medium & supplement sold together packaged separately | Size: Yields 250 ml | CAT.#: 615K-250 | Price: $87.00 | |
| Human Blood Cell Basal Medium: Basal medium (contains no growth supplement). Add Supplement before use. | Size: 250 ml | CAT.#: 614-250 | Price: $49.00 | |
| Human Blood Cell Culture Supplement: Added to Basal Medium to create Culture (Maintenance) Medium | Size: 40 ml | CAT.#: 615-CS | Price: $52.00 | |
| Freezing Medium for Blood Cells: Freezing Medium optimized for the unique needs of Blood Cells | Size: 50 ml | CAT.#: 040-50BC | Price: $75.00 |
Extended Family Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Rabbit B Cell Activating Factor Receptor Anitbody: Rabbit B Cell Activating Factor Receptor Anitbody | Size: 100 ul | CAT.#: CA1391 | Price: $302.00 | |
| Human BAFF ELISA Kit: Human B Cell Activating Factor ELISA Kit | Size: 96 Wells | CAT.#: CL0663 | Price: $533.00 | |
| Mouse BAFF ELISA Kit: Mouse B Cell Activating Factor ELISA | Size: 96 Wells | CAT.#: CL0664 | Price: $619.00 | |
| Human B cell Activating Factor (BAFF/BLyS): Human B cell Activating Factor | Size: 20 ug | CAT.#: RP1024-20 | Price: $194.00 | |
| Human B cell Activating Factor (BAFF/BLyS): Human B cell Activating Factor | Size: 100 ug | CAT.#: RP1024-100 | Price: $624.00 | |
| Human B cell Activating Factor (BAFF/BLyS): Human B cell Activating Factor | Size: 1000 ug | CAT.#: RP1024-1000 | Price: $5,327.00 | |
| Human FMS-Related Tyrosine Kinase 3 Ligand (FLT-3 ligand): Human FMS-related Tyrosine Kinase 3 Ligand | Size: 10 ug | CAT.#: RP1021-10 | Price: $194.00 | |
| Freezing Medium for Blood Cells: Freezing Medium optimized for the unique needs of Blood Cells | Size: 50 ml | CAT.#: 040-50BC | Price: $75.00 | |
| Human FMS-Related Tyrosine Kinase 3 Ligand (FLT-3 ligand): Human FMS-related Tyrosine Kinase 3 Ligand | Size: 100 ug | CAT.#: RP1021-100 | Price: $484.00 | |
| Human FMS-Related Tyrosine Kinase 3 Ligand (FLT-3 ligand): Human FMS-related Tyrosine Kinase 3 Ligand | Size: 1000 ug | CAT.#: RP1021-1000 | Price: $4,090.00 | |
| Mouse Epstein-Barr Virus Induced Gene 3 (EBI3 subunit / IL-27 / IL-35): Mouse EBV Induced Gene-3 | Size: 10 ug | CAT.#: RP2057-10 | Price: $194.00 | |
| Mouse Epstein-Barr Virus Induced Gene 3 (EBI3 subunit / IL-27 / IL-35): Mouse EBV Induced Gene-3 | Size: 100 ug | CAT.#: RP2057-100 | Price: $624.00 | |
| Mouse Epstein-Barr Virus Induced Gene 3 (EBI3 subunit / IL-27 / IL-35): Mouse EBV Induced Gene-3 | Size: 1000 ug | CAT.#: RP2057-1000 | Price: $5,327.00 | |
| Human L-Selectin ELISA Kit: Human L-Selectin ELISA Kit | Size: 96 wells | CAT.#: CL0503 | Price: $500.00 | |
| Human Macrophage Inflammatory Protein-3 Alpha (MIP-3 alpha/CCL20): Human Macrophage Inflammatory Protein-3 alpha | Size: 20 ug | CAT.#: RP1147-20 | Price: $194.00 | |
| Human Macrophage Inflammatory Protein-3 Alpha (MIP-3 alpha/CCL20): Human Macrophage Inflammatory Protein-3 alpha | Size: 100 ug | CAT.#: RP1147-100 | Price: $484.00 | |
| Human Macrophage Inflammatory Protein-3 Alpha (MIP-3 alpha/CCL20): Human Macrophage Inflammatory Protein-3 alpha | Size: 1000 ug | CAT.#: RP1147-1000 | Price: $3,175.00 | |
| Human MIP-3a ELISA Kit: Human Macrophage Inflammatory Protein-3 alpha ELISA Kit | Size: 96 Wells | CAT.#: CL0453 | Price: $495.00 | |
| Human FLT-3 ligand, Animal-Free: Human FMS-Related Tyrosine Kinase 3 Ligand, Animal-Free | Size: 10 ug | CAT.#: RP1021AF-10 | Price: $213.00 | |
| Human FLT-3 ligand, Animal-Free: Human FMS-Related Tyrosine Kinase 3 Ligand, Animal-Free | Size: 100 ug | CAT.#: RP1021AF-100 | Price: $533.00 | |
| Human FLT-3 ligand, Animal-Free: Human FMS-Related Tyrosine Kinase 3 Ligand, Animal-Free | Size: 1000 ug | CAT.#: RP1021AF-1000 | Price: $4,499.00 |
Resources/Documents
Citations
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2016
Teramura, Y., S. Asif, K. Ekdahl, E. Gustafson and B. Nilsson. 2016. Cell adhesion induced using surface modification with cell-penetrating peptide-conjugated PEG-lipid: A new cell glue for 3D cell-based structures. ACS Appl Mater Interfaces, DOI: 10.1021/acsami.6b14584.
2011
Yen, T.-Y., B.A. Macher, C.A. McDonald, C. Alleyne-Chin, and L.C. Timpe. 2011. Glycoprotein Profiles of Human Breast Cells Demonstrate a Clear Clustering of Normal/Benign versus Malignant Cell Lines and Basal versus Luminal Cell Lines. Journal of proteome research. 11:656-667.