MSDS Cryopreserved Cells
Instructions HPASMC
Cell Apps Flyer Smooth Muscle Cells
Cell Apps Flyer Cardiovascular Cells
Cell Apps Flyer Airway Cells
5 Important Cell Culture Rules
Cell Apps Poster Primary Cells
Description
Human Pulmonary Artery Smooth Muscle Cells (HPASMC) from Cell Applications, Inc. have been used to show that:
- Antitumor drugs can selectively target remodeled pulmonary vessels, but not normal vessels
- Serotonin induces their growth and activates the BMP receptor
- IL-22 promotes the growth of pulmonary vascular SMCs via a signaling mechanism that involves NADPH oxidase-dependent oxidation
- Inducers of pulmonary hypertension, characterized by thickened pulmonary arterial walls, can be suppressed by targeting gene transcription
- Cell growth can be suppressed by trans-retinoic acid by inducing expression of a known cell growth suppressor,
- Retinoic acid and its receptors may be involved in cell growth and pulmonary vascular remodeling
- Iron chelation exerts effects on vascular remodeling and is implicated for development of pulmonary hypertension as a result of ROS activity
Characterization: positive for smooth muscle cell specific alpha-actin expression
(Click to Enlarge) Human Pulmonary Artery Smooth Muscle Cells: HPASMC. Cells isolated from the vessel that carries deoxygenated blood from the heart to the lungs.
Details
Tissue | Normal healthy human pulmonary artery |
QC | No bacteria, yeast, fungi, mycoplasma, virus |
Character | Smooth muscle specific α-actin positive |
Bioassay | Attach, spread, proliferate in Growth Med |
Cryovial | 500,000 HPASMC frozen in Basal Medium w/ 10% FBS, 10% DMSO |
Kit | Cryovial frozen HPASMC (352-05a), Growth Medium (311-500), Subcltr Rgnt Kit (090K) |
Proliferating | Shipped in Gr Med, 3rd psg (flasks or plates) |
Doublings | At least 16 |
Applications | Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use. |
Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Cryopreserved Human Pulmonary Artery Smooth Muscle Cells Total Kit, adult: 5x10^5 Cells (Adult), Medium & Subculture Reagents (See Details tab for specifics) | Size: 1 Kit | CAT.#: 352K-05a | Price: $899.00 | |
| Cryopreserved Pulmonary Artery Smooth Muscle Cells (HPASMC), adult: Frozen HPASMC (5x10^5) | Size: 1 Cryovial | CAT.#: 352-05a | Price: $725.00 | |
| Proliferating Pulmonary Artery Smooth Muscle Cells (HPASMC), adult: Actively growing, dividing cells in medium | Size: T-25 Flask | CAT.#: 353-25a | Price: $725.00 | |
| Proliferating Pulmonary Artery Smooth Muscle Cells (HPASMC), adult: Actively growing, dividing cells in medium | Size: T-75 Flask | CAT.#: 353-75a | Price: $915.00 | |
| Proliferating Pulmonary Artery Smooth Muscle Cells (HPASMC), adult: Actively growing, dividing cells in medium | Size: 24 Well | CAT.#: 353-24W | Price: $915.00 | |
| Proliferating Pulmonary Artery Smooth Muscle Cells (HPASMC), adult: Actively growing, dividing cells in medium | Size: 96 Well | CAT.#: 353-96W | Price: $1,035.00 |
Related Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Human SMC Basal Medium: Basal medium (contains no growth supplement). Add GS before use. | Size: 500 ml | CAT.#: 310-500 | Price: $74.00 | |
| Human SMC Differentiation Medium: Promotes cells to change from one type to another, more specialized | Size: 250 ml | CAT.#: 311D-250 | Price: $84.00 | |
| Human SMC Growth Medium: All-in-one ready-to-use | Size: 500 ml | CAT.#: 311-500 | Price: $121.00 | |
| Human SMC Growth Medium Kit: Basal medium & growth supplement sold together packaged separately | Size: Yields 500ml | CAT.#: 311K-500 | Price: $130.00 | |
| Human SMC Growth Supplement: Added to Basal Medium to create Growth Medium | Size: 30 ml | CAT.#: 311-GS | Price: $74.00 | |
| Subculture Reagent Kit: 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution | Size: 1 Kit | CAT.#: 090K | Price: $63.00 |
Extended Family Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Mouse Interleukin-6 Antibody: Mouse Interleukin-6 Antibody | Size: 100 ul | CAT.#: CP10325 | Price: $302.00 | |
| Cyto-X Cell Counting Reagent: 500 tests | Size: 1 Bottle | CAT.#: 028-01 | Price: $160.00 | |
| Cyto-X Cell Counting Reagent Sample: 100 tests | Size: Sample | CAT.#: 028-S | Price: $41.00 | |
| Cytofect Smooth Muscle Cell Transfection Kit (125 x 24-Wells): 125 x 24-Well | Size: 1 Kit | CAT.#: TF350K | Price: $328.00 | |
| Cytofect Smooth Muscle Cell Transfection Sample Kit (25 x 24-Wells): 25 x 24-Well Rxns | Size: 1 Sample Kit | CAT.#: TF350KS | Price: $62.00 | |
| Human Heart RNA: Total RNA prepared from human heart tissue | Size: 50 ug | CAT.#: 1H30-50 | Price: $158.00 | |
| Human Heart RNA: Total RNA prepared from human heart tissue | Size: 250 ug | CAT.#: 1H30-250 | Price: $595.00 | |
| Pulmonary Artery Smooth Muscle Cell RNA (HPASMC RNA), Adult: Total RNA prepared from Human Pulmonary Artery Smooth Muscle Cells, adult | Size: 10 ug | CAT.#: 352-R10a | Price: $398.00 | |
| Pulmonary Artery Smooth Muscle Cell RNA (HPASMC RNA), Adult: Total RNA prepared from Human Pulmonary Artery Smooth Muscle Cells, adult | Size: 25 ug | CAT.#: 352-R25a | Price: $796.00 | |
| Human Interleukin-6 (IL-6): Human Interleukin-6 | Size: 20 ug | CAT.#: RP1010-20 | Price: $194.00 | |
| Human Interleukin-6 (IL-6): Human Interleukin-6 | Size: 100 ug | CAT.#: RP1010-100 | Price: $484.00 | |
| Human Interleukin-6 (IL-6): Human Interleukin-6 | Size: 1000 ug | CAT.#: RP1010-1000 | Price: $3,175.00 | |
| Human IL-6 ELISA Kit: Human Interleukin-6 ELISA Kit | Size: 96 Wells | CAT.#: CL0410 | Price: $517.00 | |
| Human IL-6, Animal Free: Human Interleukin-6, Animal-Free | Size: 20 ug | CAT.#: RP1010AF-20 | Price: $213.00 | |
| Human IL-6, Animal Free: Human Interleukin-6, Animal-Free | Size: 100 ug | CAT.#: RP1010AF-100 | Price: $533.00 | |
| Human IL-6, Animal Free: Human Interleukin-6, Animal-Free | Size: 1000 ug | CAT.#: RP1010AF-1000 | Price: $3,492.00 |
Resources/Documents
Citations
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2016
Sahoo, S., D. Meijles, I. Ghouleh, M. Tandon, E. Cifuentes-Pagano, J. Sembrat, M. Rojas, E. Goncharova and P. Pagano. 2016. MEF2C-MYOCD and Leiomodin1 Suppression by miRNA-214 Promotes Smooth Muscle Cell Phenotype Switching in Pulmonary Arterial Hypertension. PLoS ONE, 11(5):e013780.
2015
Zisman, L. 2015. Therapeutic Indications of Kinase Inhibitors. Patent Application 20150352111 A1.
2014
Ibrahim, Y., C. Wong, L. Pavlickova, L. Liu, L. Trasar, G. Bansal, and Y. Suzuki. 2014. Mechanism of the Susceptibility of Remodeled Pulmonary Vessels to Drug‐Induced Cell Killing. J Am Heart Assoc, 3: e000520. Feb 26.
Zisman, L. 2014. Non-selective kinase inhibitors. Patent Application US 20150353527 A1.
2013
Bansal, G., D. Das, C.-Y. Hsieh, Y.-H. Wang, B.A. Gilmore, C.-M. Wong, and Y.J. Suzuki. 2013. IL-22 activates oxidant signaling in pulmonary vascular smooth muscle cells. Cellular signalling. 25:2727-2733.
2012
Bansal, G., C. Wong, L. Liu and Y. Suzuki. 2012. Oxidant signaling for interleukin-13 gene expression in lung smooth muscle cells. Free Rad Biol & Med, 52:1552-1559.
Wong, C., I. Preston, N. Hill and Y. Suzuki. 2012. Iron chelation inhibits the development of pulmonary vascular remodeling. Free Rad Biol & Med, 53:1738-1747.
2009
Day, R.M., I.A. Matus, Y.J. Suzuki, K.J. YEUM, J. Qin, A.M. PARK, V. Jain, T. Kuru, and G. Tang. 2009. Plasma levels of retinoids, carotenoids and tocopherols in patients with mild obstructive sleep apnoea. Respirology. 14:1134-1142.
Liu, Y., W. Ren, R. Warburton, D. Toksoz, and B.L. Fanburg. 2009. Serotonin induces Rho/ROCK-dependent activation of Smads 1/5/8 in pulmonary artery smooth muscle cells. The FASEB Journal. 23:2299-2306.
2007
Suzuki, Y.J., H. Nagase, C.M. Wong, S.V. Kumar, V. Jain, A.-M. Park, and R.M. Day. 2007. Regulation of Bcl-xL expression in lung vascular smooth muscle. American journal of respiratory cell and molecular biology. 36:678.
2006
Behera, A.K., E. Hildebrand, J. Szafranski, H.H. Hung, A.J. Grodzinsky, R. Lafyatis, A.E. Koch, R. Kalish, G. Perides, and A.C. Steere. 2006. Role of aggrecanase 1 in Lyme arthritis. Arthritis & Rheumatism. 54:3319-3329.
Day, R.M., Y.H. Lee, A.M. Park, and Y.J. Suzuki. 2006. Retinoic acid inhibits airway smooth muscle cell migration. American journal of respiratory cell and molecular biology. 34:695-703.
2005
Preston, I.R., G. Tang, J.U. Tilan, N.S. Hill, and Y.J. Suzuki. 2005. Retinoids and pulmonary hypertension. Circulation. 111:782-790.