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5 Important Cell Culture Rules
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Description
Human Renal Proximal Tubular Epithelial Cells (HRPTEpC) provide a useful tool for studying various aspects of pathology and biology of human kidney cells in vitro. HRPTEpC from Cell Applications, Inc. have recently been used in a study of metabolic enzymes responding to energy depletion in renal cells, and they a pivotal role in kidney function including reabsorbtion of glucose and protein, and also play a part in the glucotoxicity associated with diabetes.
Characterization: Morphology consistent with epithelial origin, and positive for epithelial cell marker cytokeratin 18
(Click to Enlarge) Human Renal Proximal Tubular Epithelial Cells, HRPTEpC, Confocal image (L) and immunofluorescently stained with anti-CK18 (R)
Details
| Tissue | Normal healthy human Kidney |
| QC | No bacteria, yeast, fungi, mycoplasma, virus |
| Bioassay | Attach, spread, proliferate in Growth Med |
| Cryovial | 500,000 HRPTEpC (1st or 2nd psg) frozen in Basal Med w/ 10% FBS, 10% DMSO |
| Kit | Cryovial frozen HRPTEpC (930-05a), Growth Medium (911-500), Subcltr Rgnt Kit (090K) |
| Proliferating | Shipped in Tsfr Med, psg 3, flasks or plates |
| Doublings | At least 6 |
| Applications | Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use. |
Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Cryopreserved Human Renal Proximal Tubular Epithelial Cells Total Kit, adult: 5x10^5 Cells (Adult), Medium & Subculture Reagents (See Details tab for specifics) | Size: 1 Kit | CAT.#: 930K-05a | Price: $911.00 | |
| Cryopreserved Renal Proximal Tubular Epithelial Cells (HRPTEpC), adult: Frozen HRPTEpC (5x10^5) | Size: 1 Cryovial | CAT.#: 930-05a | Price: $715.00 | |
| Proliferating Renal Proximal Tubular Epithelial Cells (HRPTEpC), adult: Actively growing, dividing cells in medium | Size: T-25 Flask | CAT.#: 931-25a | Price: $715.00 | |
| Proliferating Renal Proximal Tubular Epithelial Cells (HRPTEpC), adult: Actively growing, dividing cells in medium | Size: T-75 Flask | CAT.#: 931-75a | Price: $905.00 | |
| Proliferating Renal Proximal Tubular Epithelial Cells (HRPTEpC), adult: Actively growing, dividing cells in medium | Size: 96 Well | CAT.#: 931-96Wa | Price: $1,025.00 | |
| Cryopreserved Human Renal Proximal Tubular Epithelial Cells Total Kit, fetal: 5x10^5 Cells (Fetal), Medium & Subculture Reagents (See Details tab for specifics) | Size: 1 Kit | CAT.#: 930K-05f | Price: $911.00 | |
| Cryopreserved Renal Proximal Tubular Epithelial Cells (HRPTEpC), fetal: Frozen HRPTEpC (5x10^5) | Size: 1 Cryovial | CAT.#: 930-05f | Price: $715.00 | |
| Proliferating Renal Proximal Tubular Epithelial Cells (HRPTEpC), fetal: Actively growing, dividing cells in medium | Size: T-25 Flask | CAT.#: 931-25f | Price: $715.00 | |
| Proliferating Renal Proximal Tubular Epithelial Cells (HRPTEpC), fetal: Actively growing, dividing cells in medium | Size: T-75 Flask | CAT.#: 931-75f | Price: $905.00 | |
| Proliferating Renal Proximal Tubular Epithelial Cells (HRPTEpC), fetal: Actively growing, dividing cells in medium | Size: 96 Well | CAT.#: 931-96Wf | Price: $1,025.00 | |
| Cryopreserved Human Renal Proximal Tubular Epithelial Cells Total Kit, neonatal: 5x10^5 Cells (Neonatal), Medium & Subculture Reagents (See Details tab for specifics) | Size: 1 Kit | CAT.#: 930K-05n | Price: $911.00 | |
| Cryopreserved Renal Proximal Tubular Epithelial Cells (HRPTEpC), neonatal: Frozen HRPTEpC (5x10^5) | Size: 1 Cryovial | CAT.#: 930-05n | Price: $715.00 | |
| Proliferating Renal Proximal Tubular Epithelial Cells (HRPTEpC), neonatal: Actively growing, dividing cells in medium | Size: T-25 Flask | CAT.#: 931-25n | Price: $715.00 | |
| Proliferating Renal Proximal Tubular Epithelial Cells (HRPTEpC), neonatal: Actively growing, dividing cells in medium | Size: T-75 Flask | CAT.#: 931-75n | Price: $905.00 | |
| Proliferating Renal Proximal Tubular Epithelial Cells (HRPTEpC), neonatal: Actively growing, dividing cells in medium | Size: 96 Well | CAT.#: 931-96Wn | Price: $1,025.00 |
Related Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Human RenaEpi Basal Medium: Basal medium (contains no growth supplement). Add GS before use. | Size: 500 ml | CAT.#: 910-500 | Price: $85.00 | |
| Human RenaEpi Growth Medium: All-in-one ready-to-use | Size: 500 ml | CAT.#: 911-500 | Price: $143.00 | |
| Human RenaEpi Growth Medium Kit: Basal medium & growth supplement sold together packaged separately | Size: Yields 500 ml | CAT.#: 911K-500 | Price: $155.00 | |
| Human RenaEpi Growth Supplement: Added to Basal Medium to create Growth Medium | Size: 10 ml | CAT.#: 911-GS | Price: $91.00 |
Extended Family Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Monoclonal IL-1 Receptor Antagonist Antibody: Monoclonal IL-1 Receptor Antagonist Antibody | Size: 100 ul | CAT.#: CB19846 | Price: $302.00 | |
| Freezing Medium: For general cryopreservation of most primary cells. Contains FBS & DMSO. | Size: 50 ml | CAT.#: 040-50 | Price: $54.00 | |
| Cyto-X Cell Counting Reagent: 500 tests | Size: 1 Bottle | CAT.#: 028-01 | Price: $160.00 | |
| Cyto-X Cell Counting Reagent Sample: 100 tests | Size: Sample | CAT.#: 028-S | Price: $41.00 | |
| Cytofect Epithelial Cell Transfection Kit (124 x 24-Wells): 124 x 24-Well Rxns | Size: 1 Kit | CAT.#: TF102K | Price: $465.00 | |
| Cytofect Epithelial Cell Transfection Sample Kit (25 x 24-Wells): 25 x 24-Well Rxns | Size: 1 Sample Kit | CAT.#: TF102KS | Price: $62.00 | |
| Renal Proximal Tubule Epithelial Cell RNA (HRPTEpC RNA), Adult: Total RNA prepared from Human Renal Proximal Tubular Epithelial Cells, adult | Size: 10 ug | CAT.#: 930-R10a | Price: $514.00 | |
| Renal Proximal Tubule Epithelial Cell RNA (HRPTEpC RNA), Adult: Total RNA prepared from Human Renal Proximal Tubular Epithelial Cells, adult | Size: 25 ug | CAT.#: 930-R25a | Price: $1,028.00 | |
| Renal Proximal Tubule Epithelial Cell RNA (HRPTEpC RNA), Fetal: Human Renal Proximal Tubular Epithelial Cells RNA, fetal | Size: 10 ug | CAT.#: 930-R10f | Price: $465.00 | |
| Renal Proximal Tubule Epithelial Cell RNA (HRPTEpC RNA), Fetal: Total RNA prepared from Human Renal Proximal Tubular Epithelial Cells, fetal | Size: 25 ug | CAT.#: 930-R25f | Price: $930.00 | |
| Human IL-1 alpha ELISA Kit: Human Interleukin-1 alpha ELISA Kit | Size: 96 wells | CAT.#: CL0389 | Price: $484.00 | |
| Human Interleukin-1-Alpha (IL-1 alpha): Human Interleukin-1-alpha | Size: 10 ug | CAT.#: RP1173-10 | Price: $194.00 | |
| Human Interleukin-1-Alpha (IL-1 alpha): Human Interleukin-1-alpha | Size: 100 ug | CAT.#: RP1173-100 | Price: $624.00 | |
| Human Interleukin-1-Alpha (IL-1 alpha): Human Interleukin-1-alpha | Size: 1000 ug | CAT.#: RP1173-1000 | Price: $5,327.00 | |
| Human IL-1 beta ELISA Kit: Human Interleukin-1 beta ELISA Kit | Size: 96 wells | CAT.#: CL0392 | Price: $587.00 | |
| Human Kidney RNA: Total RNA prepared from human kidney tissue | Size: 50 ug | CAT.#: 1H50-50 | Price: $158.00 | |
| Human Kidney RNA: Total RNA prepared from human kidney tissue | Size: 250 ug | CAT.#: 1H50-250 | Price: $595.00 | |
| Subculture Reagent Kit: 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution | Size: 1 Kit | CAT.#: 090K | Price: $63.00 |
Resources/Documents
Citations
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2017
Bidwell, G., F. Mahdi, Q. Shao, O. Logue, J. Waller, C. Reese and A. Chade. 2017. A kidney-selective biopolymer for targeted drug delivery. Am J Physiol Renal Physiol, 312: F54-F64.
2016
Ito, H., O. Ichiyanagi, S. Naito, V. Bilim, Y. Tomita, Y. Kato, A. Nagaoka and N. Tsuchiya. 2016. GSK-3 directly regulates phospho-4EBP1 in renal cell carcinoma cell-line: an intrinsic subcellular mechanism for resistance to mTORC1 inhibition. BMC Cancer 16:393.
Zhou, X., R. Liu, S. Duan, G. Huang, Y. Ye and Y. Kong. 2016. High Glucose Enhances oxLDL-Induced Apoptosis in Human Renal Proximal Tubular Epithelial Cells Largely via Inducing Lectin-Like ox-LDL Receptor-1. Pharmacology, 98:20-28.
2014
Jackson, E., D. Gillespie, Z. Mi, D. Cheng, R. Bansal, K. Janesko-Feldman and P. Kochanek. 2014.Role of 2′,3′-cyclic nucleotide 3′-phosphodiesterase in the renal 2′,3′-cAMP-adenosine pathway. Am J Physiol – Renal Physiol, 307:F14-F24.
2012
Jackson, E. and D. Gillespie. 2012. Extracellular 2′,3′-cAMP and 3′,5′-cAMP stimulate proliferation of preglomerular vascular endothelial cells and renal epithelial cells. Am J Physiol – Renal Physiol, 303:F954-F962.