Instructions HTEpC Normal
5 Important Cell Culture Rules
MSDS Cryopreserved Cells
Cell Apps Flyer Epithelial Cells
Cell Apps Poster Primary Cells
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Description
Human Tracheal Epithelial Cells (HTEpC) provide a useful model system to study all aspects of epithelial function and disease, particularly those related to airway viral infections, as well as tissue repair mechanisms, signaling changes and potential treatments relevant to lung injuries, mechanical and oxidative stress, pulmonary diseases and smoking. Note that Cell Applications, Inc. offers HTEpC obtained from healthy donors, as well as from asthma patients.
HTEpC from Cell Applications, Inc. have been used in a study investigating the role of goblet cell hyperplasia, possibly mediated by the expression of SPDEF, to the pathogenesis of chronic pulmonary diseases.
Characterization: Morphology consistent with epithelial origin, and positive for epithelial cell marker cytokeratin 18
(Click to Enlarge) Top: Human Tracheal Epithelial Cells: HTEpC (L). HTEpC immunolabeled with an anti-CK18 antibody (R). Bottom: HTEpC (L) transfected with GFP plasmid DNA using the Cytofect™ Epithelial Cell Transfection Kit (R).
Details
Tissue | Surface epithelium of normal healthy human trachea |
QC | No bacteria, yeast, fungi, mycoplasma, virus |
Bioassay | Attach, spread, proliferate in Growth Medium |
Cryovial | 500,000 HTEpC (1st passage) frozen in Basal Medium w/ 10% FBS, 10% DMSO |
Kit | Cryovial frozen HTEpC (504-05a), Growth Medium (511-500), Subcltr Rgnt Kit (090K) |
Proliferating | Shipped in Gr Med, 2nd psg (flasks or plates) |
Doublings | At least 16 |
Applications | Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use. |
Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Cryopreserved Human Tracheal Epithelial Cells Total Kit: 5x10^5 Cells (Adult), Medium & Subculture Reagents (See Details tab for specifics) | Size: 1 Kit | CAT.#: 504K-05a | Price: $938.00 | |
| Cryopreserved Tracheal Epithelial Cells (HTEpC), adult: Frozen HTEpC (5x10^5) | Size: 1 Cryovial | CAT.#: 504-05a | Price: $740.00 | |
| Cryopreserved Human Tracheal Epithelial Cells, with Asthma Total Kit: 5x10^5 Cells (from donor with Asthma, Adult), Medium & Subculture Reagents (See Details tab for specifics) | Size: 1 Kit | CAT.#: 504ASK-05a | Price: $1,113.00 | |
| Cryopreserved Tracheal Epithelial Cells, Asthma (HTEpC-AS), adult: Frozen HTEpC-AS from donor with Asthma | Size: 1 Cryovial | CAT.#: 504AS-05a | Price: $975.00 | |
| Proliferating Tracheal Epithelial Cells (HTEpC), adult: Actively growing, dividing cells in medium | Size: T-25 Flask | CAT.#: 505-25a | Price: $740.00 | |
| Proliferating Tracheal Epithelial Cells (HTEpC), adult: Actively growing, dividing cells in medium | Size: T-75 Flask | CAT.#: 505-75a | Price: $930.00 | |
| Proliferating Tracheal Epithelial Cells (HTEpC), adult: Actively growing, dividing cells in medium | Size: 96 Well | CAT.#: 505-96Wa | Price: $1,050.00 |
Related Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| HBrEpC/HTrEpC Basal Medium: Without growth supplement (GS). Add GS before use. | Size: 500 ml | CAT.#: 510-500 | Price: $81.00 | |
| HBEpC/HTEpC Differentiation Medium: Promotes cells to change from one type to another, more specialized | Size: 250 ml | CAT.#: 511D-250 | Price: $148.00 | |
| HBEpC/HTEpC Growth Medium: All-in-one ready-to-use. Cat# 511-500 fully supports cell growth on its own. Retinoic acid (RA) is a optional component; one vial of RA goes into 500ml media. Some researchers may elect to include RA if studies include cell differentiation or pseudo-stratification. | Size: 500 ml | CAT.#: 511-500 | Price: $145.00 | |
| HBEpC/HTEpC Growth Medium wo BPE: Growth medium without BPE | Size: 500 ml | CAT.#: 511B-500 | Price: $144.00 | |
| HBEpC/HTEpC Growth Medium wo BPE, Hydrocortisone, Insulin: Growth medium without BPE, hydrocortisone and insulin | Size: 500 ml | CAT.#: 511BHI-500 | Price: $144.00 | |
| HBEpC/HTEpC Growth Medium wo T3: Growth medium without Tri-iodothyronine (T3) | Size: 500 ml | CAT.#: 511T3-500 | Price: $144.00 | |
| HBEpC/HTEpC Growth Medium Kit: Basal medium & growth supplement sold together packaged separately | Size: 1 Kit | CAT.#: 511K-500 | Price: $154.00 | |
| HBEpC/HTEpC Growth Supplement: Added to Basal Medium to create Growth Medium | Size: 5 ml | CAT.#: 511-GS | Price: $81.00 | |
| HBEpC/HTEpC Growth Medium wo Antibiotics: Growth medium without antibiotics | Size: 500 ml | CAT.#: 511A-500 | Price: $144.00 | |
| Human EpiVita Basal Medium: Basal medium (contains no growth supplement). Add GS before use. | Size: 500 ml | CAT.#: 140-500 | Price: $96.00 |
Extended Family Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Monoclonal IL-1 Receptor Antagonist Antibody: Monoclonal IL-1 Receptor Antagonist Antibody | Size: 100 ul | CAT.#: CB19846 | Price: $302.00 | |
| Freezing Medium: For general cryopreservation of most primary cells. Contains FBS & DMSO. | Size: 50 ml | CAT.#: 040-50 | Price: $54.00 | |
| Cyto-X Cell Counting Reagent: 500 tests | Size: 1 Bottle | CAT.#: 028-01 | Price: $160.00 | |
| Cyto-X Cell Counting Reagent Sample: 100 tests | Size: Sample | CAT.#: 028-S | Price: $41.00 | |
| Cytofect Epithelial Cell Transfection Kit (124 x 24-Wells): 124 x 24-Well Rxns | Size: 1 Kit | CAT.#: TF102K | Price: $465.00 | |
| Cytofect Epithelial Cell Transfection Sample Kit (25 x 24-Wells): 25 x 24-Well Rxns | Size: 1 Sample Kit | CAT.#: TF102KS | Price: $62.00 | |
| Human Epidermal Growth Factor (EGF): Human Epidermal Growth Factor | Size: 100 ug | CAT.#: RP1026-100 | Price: $86.00 | |
| Human Epidermal Growth Factor (EGF): Human Epidermal Growth Factor | Size: 500 ug | CAT.#: RP1026-500 | Price: $194.00 | |
| Human Epidermal Growth Factor (EGF): Human Epidermal Growth Factor | Size: 1000 ug | CAT.#: RP1026-1000 | Price: $264.00 | |
| Tracheal Epithelial Cell RNA (HTEpC RNA), Adult: Total RNA prepared from Human Tracheal Epithelial Cells, adult | Size: 10 ug | CAT.#: 504-R10a | Price: $398.00 | |
| Tracheal Epithelial Cell RNA (HTEpC RNA), Adult: Total RNA prepared from Human Tracheal Epithelial Cells, adult | Size: 25 ug | CAT.#: 504-R25a | Price: $796.00 | |
| Human IL-1 alpha ELISA Kit: Human Interleukin-1 alpha ELISA Kit | Size: 96 wells | CAT.#: CL0389 | Price: $484.00 | |
| Human Interleukin-1-Alpha (IL-1 alpha): Human Interleukin-1-alpha | Size: 10 ug | CAT.#: RP1173-10 | Price: $194.00 | |
| Human Interleukin-1-Alpha (IL-1 alpha): Human Interleukin-1-alpha | Size: 100 ug | CAT.#: RP1173-100 | Price: $624.00 | |
| Human Interleukin-1-Alpha (IL-1 alpha): Human Interleukin-1-alpha | Size: 1000 ug | CAT.#: RP1173-1000 | Price: $5,327.00 | |
| Human IL-1 beta ELISA Kit: Human Interleukin-1 beta ELISA Kit | Size: 96 wells | CAT.#: CL0392 | Price: $587.00 | |
| Subculture Reagent Kit: 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution | Size: 1 Kit | CAT.#: 090K | Price: $63.00 | |
| Human EGF, Animal-Free: Human Epidermal Growth Factor, Animal-Free | Size: 100 ug | CAT.#: RP1026AF-100 | Price: $95.00 | |
| Human EGF, Animal-Free: Human Epidermal Growth Factor, Animal-Free | Size: 500 ug | CAT.#: RP1026AF-500 | Price: $213.00 | |
| Human EGF, Animal-Free: Human Epidermal Growth Factor, Animal-Free | Size: 1000 ug | CAT.#: RP1026AF-1000 | Price: $290.00 |
Resources/Documents
Citations
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2017
Chakraborty, S., V. Castranova, M. Perez and G. Piedimonte. 2017. Nanoparticles-induced apoptosis of human airway epithelium is mediated by proNGF/p75NTR signaling. J Toxicol & Env Hlth, Pt A, 80:53-68.
2016
Liu, Z., X. Chen, Q. Wu, J. Song, L. Wang and G. Li. 2016.miR-125b inhibits goblet cell differentiation in allergic airway inflammation by targeting SPDEF. Eur J Pharmacol, 782:14-20.
2007
Park, K.-S., T.R. Korfhagen, M.D. Bruno, J.A. Kitzmiller, H. Wan, S.E. Wert, G.K. Khurana Hershey, G. Chen, and J.A. Whitsett. 2007. SPDEF regulates goblet cell hyperplasia in the airway epithelium. J. clinical investigation. 117:978-988.
2006
Whitsett, J., and J. Wells. 2006. Regulatory proteins in lung repair and treatment of lung disease. Patent Application US 20110177996 A1.