MSDS Cryopreserved Cells
Instructions HCvEpC
Cell Apps Flyer Epithelial Cells
5 Important Cell Culture Rules
Cell Apps Poster Primary Cells
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Description
Human Cervical Epithelial Cells (HCvEpC) form intercellular junctions and provide structural integrity to the surface of the cervix, the lower part of the uterus in a human female reproductive system. Comprised of several layers and underlying stroma, the cervix is lined by epithelium, which secretes mucous and limits passage of infectious and invading substances. Cervical epithelial cells offer a protective environment, fortifying the barrier against infection with an autonomous, innate epithelial immunity. These defense mechanisms also include pathogen-recognition receptors, immune mediators and recruitment of effector immune cells
HCvEpC provide a useful in vitro model for studying the normal biology and pathophysiology of the human cervical epithelium. The cells have proven useful for basic life science research into cell morphology, cytoskeletal proteins & markers, differentiation, epithelial–mesenchymal transition, apoptosis, inflammation, tissue remodeling and vascularization. Others rely on the cells to better describe transcriptional regulation, gene interaction networks, signaling pathways, and ultimately proteome changes. The cells are collected during a Pap smear to detect the presence of abnormal cell growth, and are used to examine reproductive diseases, health outcomes and even response of the epithelium to sex hormones.
Researchers find HCvEpC invaluable, for instance to study diseases and pathogenesis:
- Cervical squamous intra-epithelial lesions & neoplasia
- Chromosomal instability and over-activation of telomerase activity
- Reactive oxygen species production and oxidative DNA damage
- Abnormal nuclear morphologies
- Cervical polyps
While defense mechanisms abound, the uterine cervix is susceptible to infection, for instance by chlamydia, parasites, bacteria and viruses. The tissue is also known to reveal rare neoplasms like malignant melanoma of the uterine cervix. Oncogenic virus infection is studied in cervical epithelial cells, particularly infection by human papilloma virus (HPV), which can cause epithelial changes tied to cervical cancer. Persistent high-risk HPV infection causes inflammation in the cervical epithelial barrier, precancerous lesionsand tumorigenesis.
HCvEpC have helped tease out how cervical cancer cells proliferate and metastasize. Importantly, keener understanding of cervical disease provides hope for ever better prognosis and treatment. Better markers and therapeutic targets have produced efficient, safe and well-tolerated treatments and preventive therapies. For instance, recent publications highlight advances made in topical photodynamic therapy, vitamin-induced anti-proliferative effects, polyphenolic compounds and novel approaches to control trichomoniasis. Other work describes the positive effects of inhibiting protein kinases and histone deacetylase.
Risk factors for cervical cancer have been identified, screening has improved and prophylactic HPV vaccines have been introduced. Yet physician-scientists still work toward development of therapies for HPV infections and cervical cancer, including vaccines and anti-tumor agents. Other goals include automatic detection of cervical cancer cells, and leveraging molecular biomarkers for early diagnosis of cervical lesion progression and metastasis. Such advances may afford novel targets for the prevention and therapy of cervical cancers. Approaches include tumor suppressors, inducing cancer cell senescence, down-regulating HPV gene expression, or using MicroRNAs (miRNAs), which are small non-coding RNAs, to inhibit cervical cancer cell proliferation and tumorigenesis.
Characterization: Morphology consistent with epithelial origin, and positive for epithelial cell marker cytokeratin 18
(Click to Enlarge) Top: Human Cervical Epithelial Cells: HCvEpC. Bottom: HCvEpC immunolabeled with an anti-CK18 C-terminal antibody (L). HCvEpC immunolabeled with an anti-CK19 antibody (R).
Details
Tissue | Normal healthy human cervix |
QC | No bacteria, yeast, fungi, mycoplasma, virus |
Bioassay | Attach, spread, proliferate in Growth Med |
Cryovial | 500,000 HCvEpC (2nd passage) frozen in Basal Medium w/ 10% FBS, 10% DMSO |
Kit | Cryovial frozen HCvEpC (232-05a), Growth Med (219-500), Subcltr Rgnt Kit (090K) |
Proliferating | Shipped in Tsfr Med, psg 3, flasks or plates |
Doublings | At least 6 |
Applications | Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use. |
Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Cryopreserved HCvEpC Total Kit: 5x10^5 Cells (Adult), Medium & Subculture Reagents (See Details tab for specifics) | Size: 1 Kit | CAT.#: 232K-05a | Price: $995.83 | |
| Cryopreserved HCvEpC, adult: Frozen HCvEpC (5x10^5) | Size: 1 Cryovial | CAT.#: 232-05a | Price: $839.48 | |
| Proliferating HCvEpC, adult: Actively growing, dividing cells in medium | Size: T-25 Flask | CAT.#: 233-25a | Price: $839.48 | |
| Proliferating HCvEpC, adult: Actively growing, dividing cells in medium | Size: T-75 Flask | CAT.#: 233-75a | Price: $1,043.96 | |
| Proliferating HCvEpC, adult: Actively growing, dividing cells in medium | Size: 6 Well | CAT.#: 233-6Wa | Price: $1,043.96 | |
| Proliferating HCvEpC, adult: Actively growing, dividing cells in medium | Size: 96 Well | CAT.#: 233-96Wa | Price: $1,173.11 |
Related Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Human Cervical Epithelial Cell Growth Medium: All-in-one ready-to-use | Size: 500 ml | CAT.#: 219-500 | Price: $116.24 | |
| Human Cervical Epithelial Cell Growth Medium Kit: Basal medium & growth supplement sold together packaged separately | Size: 500 ml | CAT.#: 219K-500 | Price: $127.00 | |
| Human Cervical Epithelial Cell Growth Supplement: Added to Basal Medium to create Growth Medium | Size: 5 ml (3 parts) | CAT.#: 219-GS | Price: $53.81 | |
| Human Epi Basal Medium: Basal medium (contains no growth supplement). Add GS before use. | Size: 500 ml | CAT.#: 214-500 | Price: $73.19 | |
| Human EpiVita Basal Medium: Basal medium (contains no growth supplement). Add GS before use. | Size: 500 ml | CAT.#: 140-500 | Price: $74.26 |
Extended Family Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Anti-IL-1Ra: Monoclonal IL-1 Receptor Antagonist Antibody | Size: 100 ul | CAT.#: CB19846 | Price: $302.38 | |
| Cell Freezing Medium: For general cryopreservation of most primary cells. Contains FBS & DMSO. | Size: 50 ml | CAT.#: 040-50 | Price: $53.81 | |
| Cyto-X Colorimetric Cell Counting Reagent: 500 tests | Size: 1 Bottle | CAT.#: 028-01 | Price: $138.84 | |
| Cyto-X Colorimetric Cell Counting Reagent: 100 tests | Size: Sample | CAT.#: 028-S | Price: $35.88 | |
| Cytofect™ Epithelial Cell Transfection Kit: 25 x 24-Well Rxns | Size: 1 Sample Kit | CAT.#: TF102KS | Price: $53.76 | |
| Cytofect™ Epithelial Cell Transfection Kit: 124 x 24-Well Rxns | Size: 1 Kit | CAT.#: TF102K | Price: $403.59 | |
| EGF: Human Epidermal Growth Factor | Size: 100 ug | CAT.#: RP1026-100 | Price: $86.10 | |
| EGF: Human Epidermal Growth Factor | Size: 500 ug | CAT.#: RP1026-500 | Price: $193.73 | |
| EGF: Human Epidermal Growth Factor | Size: 1000 ug | CAT.#: RP1026-1000 | Price: $263.68 | |
| EGF, Animal-Free: Human Epidermal Growth Factor, Animal-Free | Size: 100 ug | CAT.#: RP1026AF-100 | Price: $94.71 | |
| EGF, Animal-Free: Human Epidermal Growth Factor, Animal-Free | Size: 500 ug | CAT.#: RP1026AF-500 | Price: $213.10 | |
| EGF, Animal-Free: Human Epidermal Growth Factor, Animal-Free | Size: 1000 ug | CAT.#: RP1026AF-1000 | Price: $290.05 | |
| HCvEpC RNA: Total RNA prepared from Human Cervical Epithelial Cells, adult | Size: 10 ug | CAT.#: 232-R10a | Price: $452.03 | |
| HCvEpC RNA: Total RNA prepared from Human Cervical Epithelial Cells, adult | Size: 25 ug | CAT.#: 232-R25a | Price: $904.05 | |
| IL-1α / IL-1F1: Human Interleukin-1-alpha | Size: 10 ug | CAT.#: RP1173-10 | Price: $193.73 | |
| IL-1α / IL-1F1: Human Interleukin-1-alpha | Size: 100 ug | CAT.#: RP1173-100 | Price: $624.23 | |
| IL-1α / IL-1F1: Human Interleukin-1-alpha | Size: 1000 ug | CAT.#: RP1173-1000 | Price: $5,327.44 | |
| IL-1α ELISA Kit, Human: Human Interleukin-1 alpha ELISA Kit | Size: 96 wells | CAT.#: CL0389 | Price: $484.31 | |
| IL-1β ELISA Kit, Human: Human Interleukin-1 beta ELISA Kit | Size: 96 wells | CAT.#: CL0392 | Price: $586.56 | |
| Subculture Reagent Kit: 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution | Size: 1 Kit | CAT.#: 090K | Price: $54.89 |
Resources/Documents
Publications
2014
Zhao, B., X. Shang, L. Xu, W. Zhang, and G. Xiang. 2014. Novel mixed ligand di-n-butyltin(IV) complexes derived from acylpyrazolones and fluorinated benzoic acids: Synthesis, characterization, cytotoxicity and the induction of apoptosis in Hela cancer cells. European Journal of Medicinal Chemistry, 76:87–97.