Instructions RH
5 Important Cell Culture Rules
MSDS Cryopreserved Cells
Cell Apps Poster Primary Cells
Cell Applications Inc Brochure

Description

Rat Hepatocytes (RH) from CAI find uses in Phase I and Phase II work, including drug identification, stability and clearance.  Other applications include time-dependent drug metabolism, enzyme activity and drug-drug interactions.  Amenable to high content analysis, hepatocytes are employed in plated drug transporter assays (uptake and efflux), hepatotoxicity assays, inducibility studies and CYP450 Induction. The regulatory effect of insulin-like growth factor-11 on hepatocyte function and the apoptotic effect of transforming growth factor-b2 were examined in primary hepatocytes.
 
Hepatocytes, the chief functional cells of the liver, make up 70-85% of that organ’s mass.  They carryout metabolic, endocrine and secretory functions, including carbohydrate transformation and the synthesis, storage & secretion of cholesterol, bile salts, proteins, phospholipids and LDL.  Importantly, hepatocytes detoxify, modify and excrete substances. CAI hepatocytes have been used to investigate rare sugars in cancer cell growth, showing D-allose induced G1 cell cycle arrest, leading to protein stabilization and increased nuclear localization.
(Click to Enlarge) Rat Hepatocytes: RH isolated from primary liver tissue.

Details

Tissue
Normal healthy adult rat liver
QC
No bacteria, yeast, fungi, mycoplasma, virus
Bioassay
Attach to collagen-coated surface, remain viable 2-4 days in Maintenance Medium
Cryovial
3,000,000 RH (primary isolation) frozen in Maintenance Med w/ 10% FBS, 10% DMSO
Suspsn Kit
Cryovial frozen RH (R780-30n), Maintenance Medium (R713-250)
Matrix Kit
Cryovial frozen RH (R780-30n), Attachment Medium (R711-100), Matrix Extract (126-2.75)
Collagen Kit
Cryovial frozen RH (R780-30n), Attachment Medium (R711-100), Collagen Soln (122-20)
Cultured
Shipped in Maint Med (flasks or plates)
Doublings
N/A
Applications
Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use.
MSDS Cryopreserved Cells

Format: PDF

Download Now

Products

Product Size CAT.# Price Quantity
Cryopreserved RH Total Kit: 3x10^6 Cells (from Collagen, Adult), Medium & Subculture Reagents (See Details tab for specifics) Size: 1 Kit CAT.#: R780K-30ac Price: $526.77
-+
Cryopreserved RH Total Kit: 3x10^6 Cells (from suspension, Adult), Medium & Subculture Reagents (See Details tab for Specifics) Size: 1 Kit CAT.#: R780K-30as Price: $445.97
-+
Cryopreserved RH Total Kit: 3x10^6 Cells (from EHS Matrix Extract, Adult), Medium & Subculture Reagents (See Details tab for specifics) Size: 1 Kit CAT.#: R780K-30am Price: $620.16
-+
Cryopreserved RH, adult: Frozen RH (3x10^6) Size: 1 Cryovial CAT.#: R780-30a Price: $376.69
-+
Cultured RH, neonatal: RH cultured on Collagen Size: T-25 Flask CAT.#: R781c-25n Price: $376.69
-+
Cultured RH, neonatal: RH cultured on Collagen Size: T-75 Flask CAT.#: R781c-75n Price: $581.18
-+
Cultured RH, neonatal: RH cultured on Collagen Size: 6 well CAT.#: R781c-6Wn Price: $581.18
-+
Cultured RH, neonatal: RH cultured on Collagen Size: 96 Well CAT.#: R781c-96Wn Price: $710.33
-+
Cultured RH, neonatal: RH cultured on EHS Matrix Size: T-25 Flask CAT.#: R781m-25n Price: $376.69
-+
Cultured RH, neonatal: RH cultured on EHS Matrix Size: T-75 Flask CAT.#: R781m-75n Price: $581.18
-+
Cultured RH, neonatal: RH cultured on EHS Matrix Size: 6 well CAT.#: R781m-6Wn Price: $581.18
-+
Cultured RH, neonatal: RH cultured on EHS Matrix Size: 96 Well CAT.#: R781m-96Wn Price: $710.33
-+

Related Products

Product Size CAT.# Price Quantity
Rat Hepatocyte Attachment Basal Medium: Basal medium (contains no growth supplement).  Add GS before use. Size: 100 ml CAT.#: R710-100 Price: $39.82
-+
Rat Hepatocyte Attachment Medium: Rat Hepatocyte Attachment Medium Size: 100 ml CAT.#: R711-100 Price: $45.20
-+
Rat Hepatocyte Attachment Medium Kit: Rat Hepatocyte Attachment Medium Kit Size: yields 100 ml CAT.#: R711K-100 Price: $0.00
-+
Rat Hepatocyte Maintenance Basal Medium: Basal medium (contains no growth supplement).  Add GS before use. Size: 250 ml CAT.#: R712-250 Price: $53.81
-+
Rat Hepatocyte Maintenance Medium: Optimized and ready for use. Size: 250 ml CAT.#: R713-250 Price: $62.42
-+
Rat Hepatocyte Maintenance Medium Kit: Basal medium & growth supplement sold together packaged separately Size: yields 250 ml CAT.#: R713K-250 Price: $80.72
-+

Extended Family Products

Product Size CAT.# Price Quantity
Cyto-X Colorimetric Cell Counting Reagent: 500 tests Size: 1 Bottle CAT.#: 028-01 Price: $138.84
-+
Cyto-X Colorimetric Cell Counting Reagent: 100 tests Size: Sample CAT.#: 028-S Price: $35.88
-+
Cytofect™ Hepatocyte Transfection Kit: 25 x 24-Well Rxns Size: 1 Sample Kit CAT.#: TF780KS Price: $53.76
-+
Cytofect™ Hepatocyte Transfection Kit: 200 x 24-Well Rxns Size: 1 Kit CAT.#: TF780K Price: $403.59
-+
EHS Matrix Extract Concentrate: Basement membrane components extracted from Engelbreth-Holm-Swarm (EHS) mouse tumor Size: 2.5 ml CAT.#: 126-2.5 Price: $122.69
-+
Rat Tail Collagen Coating Solution: Type I Collagen Size: 20 ml CAT.#: 122-20 Price: $26.91
-+

Resources/Documents

5 Important Cell Culture Rules

Format: PDF

Downoad Now
Cell Apps Poster Primary Cells

Format: PDF

Downoad Now
Cell Applications Inc Brochure

Format: PDF

Downoad Now

Publications

2008

Yamaguchi, F., M. Takata, K. Kamitori, M. Nonaka, Y. Dong, L. Sui, and M. Tokuda. 2008. Rare sugar D-allose induces specific up-regulation of TXNIP and subsequent G1 cell cycle arrest in hepatocellular carcinoma cells by stabilization of p27kip1. International journal of oncology. 32:377.