Instructions PBMC (HMNC-PB)
5 Important Cell Culture Rules
MSDS Cryopreserved Cells
Cell Apps Flyer Cardiovascular Cells
Cell Apps Poster Primary Cells
Cell Applications Inc Brochure

Description

Peripheral Blood Mononuclear Cells (PBMC), also known as Human Mononuclear Cells from Peripheral Blood (HMNC-PB), are widely used in research and clinical applications every day, and provide a useful tool for studying various aspects of pathology and biology in vitro. Cell Applications PBMC are cryopreserved immediately after isolation.
PBMC / HMNC-PB are important tools for studies in:
  • Immunology (including auto-immune disorders), infectious disease, hematological malignancies, vaccine development, transplant immunology
  • High-throughput screening
  • Neovascularization to improve limb ischemia and in cell therapy to repair ischemic and infracted myocardium.
  • Human body defense mechanisms critical in the immune system to fight bacterial and viral infections, and function as immune surveillance network to kill tumor cells.
  • The secretion of cytokines to regulate immune response and inflammation
  • Biological effects on tissue and organs.
  • Chemokine effects on adipocytes and muscles
(Click to Enlarge) Peripheral Blood Mononuclear Cells: PBMC, also known as Human Mononuclear Cells from Peripheral Blood (HMNC-PB).

Details

Tissue
Normal healthy human peripheral blood
QC
No bacteria, yeast, fungi, mycoplasma, virus
Cryovial
10,000,000 PBMC / HMNC-PB frozen right after isolation in DMEM w/ 45% FBS, 10% DMSO
Kit
Cryovial Frozen PBMC / HMNC-PB, Culture Medium (615-250)
DoublingsN/A - Blood cells don't proliferate in vitro
  

Products

Product Size CAT.# Price Quantity
Cryopreserved Human Mononuclear Cells-Periphery Blood Total Kit, adult: 1x10^7 Cells (Adult) & Medium (See Details tab for specifics) Size: 1 Kit CAT.#: 690PBK-100a Price: $344.00
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Cryopreserved Peripheral Blood Cells (PBMC/HMNC-PB), adult: Frozen PBMC (1x10^7) Size: 1 Cryovial CAT.#: 690PB-100a Price: $278.00
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Related Products

Product Size CAT.# Price Quantity
Human Blood Cell Culture Medium: Optimized and ready for use. Size: 250 ml CAT.#: 615-250 Price: $76.00
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Human Blood Cell Culture Medium Kit: Basal medium & supplement sold together packaged separately Size: Yields 250 ml CAT.#: 615K-250 Price: $87.00
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Human Blood Cell Basal Medium: Basal medium (contains no growth supplement).  Add Supplement before use. Size: 250 ml CAT.#: 614-250 Price: $49.00
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Human Blood Cell Culture Supplement: Added to Basal Medium to create Culture (Maintenance) Medium Size: 40 ml CAT.#: 615-CS Price: $52.00
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Hematopoietic Cell Freezing Medium: Serum-free freezing medium optimized for Peripheral Blood Mononuclear Cells (PBMC) Size: 50 ml CAT.#: 041-50 Price: $85.00
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Extended Family Products

Product Size CAT.# Price Quantity
Mouse Cluster of Differentiation 3 Antibody: Mouse Cluster of Differentiation 3 Antibody Size: 100 ul CAT.#: CP10034 Price: $302.00
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Mouse Cluster of Differentiation 4 Antibody: Mouse Cluster of Differentiation 4 Antibody Size: 100 ul CAT.#: CP10035 Price: $302.00
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Mouse CD8 monoclonal Antibody: Mouse CD8 monoclonal antibody Size: 100 ul CAT.#: CP10036 Price: $302.00
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Anti-CD8: Rabbit CD8 antibody Size: 100 ul CAT.#: CG1649 Price: $289.00
Freezing Medium for Blood Cells: Freezing Medium optimized for the unique needs of Blood Cells Size: 50 ml CAT.#: 040-50BC Price: $75.00
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Human FMS-Related Tyrosine Kinase 3 Ligand (FLT-3 ligand): Human FMS-related Tyrosine Kinase 3 Ligand Size: 10 ug CAT.#: RP1021-10 Price: $194.00
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Human FMS-Related Tyrosine Kinase 3 Ligand (FLT-3 ligand): Human FMS-related Tyrosine Kinase 3 Ligand Size: 100 ug CAT.#: RP1021-100 Price: $484.00
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Human FMS-Related Tyrosine Kinase 3 Ligand (FLT-3 ligand): Human FMS-related Tyrosine Kinase 3 Ligand Size: 1000 ug CAT.#: RP1021-1000 Price: $4,090.00
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Cryopreserved Human Peripheral Blood CD19+ B Cells Total Kit, adult: 2x10^6 CD19+ B Cells (Adult) & Medium (See Details tab for specifics) Size: 1 Kit CAT.#: 6904K-20a Price: $991.00
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Cryopreserved Peripheral Blood B Cells (HPBB), adult: Frozen HPBB, CD19+ (2x10^6) Size: 1 Cryovial CAT.#: 6904-20a Price: $925.00
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Cryopreserved Human Peripheral Blood CD4+ T Cells Total Kit, adult: 5x10^6 Cells CD4+ T Cells (Adult) & Medium (See Details tab for specifics) Size: 1 Kit CAT.#: 6902K-50a Price: $891.00
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Cryopreserved Peripheral Blood T Cells (HPBT), adult: Frozen HPBT, CD4+ Size: 1 Cryovial CAT.#: 6902-50a Price: $825.00
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Cyto-X Cell Counting Reagent: 500 tests Size: 1 Bottle CAT.#: 028-01 Price: $160.00
Cyto-X Cell Counting Reagent Sample: 100 tests Size: Sample CAT.#: 028-S Price: $41.00
Human FLT-3 ligand, Animal-Free: Human FMS-Related Tyrosine Kinase 3 Ligand, Animal-Free Size: 10 ug CAT.#: RP1021AF-10 Price: $213.00
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Human FLT-3 ligand, Animal-Free: Human FMS-Related Tyrosine Kinase 3 Ligand, Animal-Free Size: 100 ug CAT.#: RP1021AF-100 Price: $533.00
-+
Human FLT-3 ligand, Animal-Free: Human FMS-Related Tyrosine Kinase 3 Ligand, Animal-Free Size: 1000 ug CAT.#: RP1021AF-1000 Price: $4,499.00
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Resources/Documents

5 Important Cell Culture Rules

Format: PDF

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Cell Apps Flyer Cardiovascular Cells

Format: PDF

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Cell Apps Poster Primary Cells

Format: PDF

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Cell Applications Inc Brochure

Format: PDF

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Publications

2017
Ota, K. and S. Tanaka. 2017. ANIMAL CELL CULTURE MEDIUM AND CULTURE CONTAINER. Patent Application 20170022477 A1.