Instructions RHbN
MSDS Cryopreserved Cells
5 Important Cell Culture Rules
Cell Apps Flyer Nervous System
Cell Apps Flyer Brain Cells
Cell Apps Poster Primary Cells
Cell Applications Inc Brochure

Description

Rat Hindbrain Neurons (RHbN) are derived from hindbrains of normal embryonic rat (gestation day 15) by standardized methods. When cultured under the recommended conditions, RHbN arborize and form complex neurite network in one week. RHbN Stain positive for β III-Tubulin.

The hindbrain includes the cerebellum, the pons, and the medulla. These parts together control and support various vital bodily processes such as breathing, swallowing, blood circulation, muscle tone, sleep and arousal patterns, coordination, equilibrium maintenance, and more. Malformation, vascular disorders, atrophies, and traumas at hindbrain strike its proper structure and functions, leading to the manifestation of a variety of clinical features of different severity.  Studying hindbrain neurons in those disease conditions will allow a better understanding of the disease mechanisms, physiopathologies, and advancement in drug development and therapies. Cultures of hindbrain neurons can be applied for a variety of experiments including toxicity test, immunostaining, live cell imaging, co-culturing, electrophysiology, and more.

(Click to Enlarge) Cryopreserved Rat Hindbrain Neurons (RHbN) revived and cultured for 8 days (top), stained (bottom) for β III-Tubulin (green), GFAP (red), and DAPI (blue).

Details

TissueNormal healthy rat hindbrain
QCNo bacteria, yeast, fungi, mycoplasma, virus  
CharacterPositive for β-III Tubulin
BioassayPlate on Poly-D-Lysine coated surface, arborize to form neurite network in Culture Med
Cryovial1,000,000 RHbN in Ser-Fr Frzng Med (042-50)
  
KitCryovial frozen RHbN, Ctng Soln I (027-05), Plating Med (R817P-10), Cultr Med (R817-100)
DoublingsN/A - Neurons don't proliferate in vitro
ApplicationsLaboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use.
Instructions RHbN

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MSDS Cryopreserved Cells

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Products

Product Size CAT.# Price Quantity
Cryopreserved Rat Hindbrain Neurons Total Kit: 1x10^6 Cells, Plating Medium, Culture Medium & Coating Solution (See Details tab for specifics) Size: 1 Kit CAT.#: R888NK-10 Price: $568.00
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Cryopreserved Rat Hindbrain Neurons (RHbN): Frozen RHbN (1x10^6) Size: 1 Cryovial CAT.#: R888N-10 Price: $465.00
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Related Products

Product Size CAT.# Price Quantity
Rat Neuron Culture Medium: For the culture and maintenance of neurons. (For rat dorsal root gangion neurons, use Cat# R823K-100.) Size: 100 ml CAT.#: R817-100 Price: $72.00
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Rat Neuron Culture Medium Kit: Basal medium & growth supplement sold together packaged separately. (For rat dorsal root gangion neurons, use Cat# R823K-100.) Size: Yields 100 ml CAT.#: R817K-100 Price: $83.00
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Rat Neuron Basal Medium: Basal medium (contains no growth supplement).  Add GS before use. Size: 100 ml CAT.#: R816-100 Price: $40.00
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Rat Neuron Culture Supplement: Added to Basal Medium to create Culture Medium Size: 3 ml CAT.#: R817-GS Price: $57.00
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Neuron Coating Solution I: Poly-D-Lysine coating for neurons Size: 5 ml CAT.#: 027-05 Price: $18.00
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Neuron Coating Solution I: Poly-D-Lysine coating for neurons Size: 10 ml CAT.#: 027-10 Price: $36.00
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Rat Neuron Plating Solution I: For initial plating of neurons Size: 10 ml CAT.#: R817P-10 Price: $23.00
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Rat Neuron Plating Solution I: For initial plating of neurons Size: 20 ml CAT.#: R817P-20 Price: $45.00
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Rat Neuron Plating Solution I: For initial plating of neurons Size: 40 ml CAT.#: R817P-40 Price: $71.00
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Extended Family Products

Product Size CAT.# Price Quantity
Cytofect Neuron Transfection Kit (100 x 24-Wells): 100 x 24-Well Rxns Size: 1 Kit CAT.#: TF886K Price: $465.00
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Cytofect Neuron Transfection Sample Kit (25 x 24-Wells): 25 x 24-Well Rxns Size: 1 Sample Kit CAT.#: TF886KS Price: $62.00
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Cyto-X Cell Counting Reagent: 500 tests Size: 1 Bottle CAT.#: 028-01 Price: $160.00
Cyto-X Cell Counting Reagent Sample: 100 tests Size: Sample CAT.#: 028-S Price: $41.00

Resources/Documents

5 Important Cell Culture Rules

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Cell Apps Flyer Nervous System

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Cell Apps Flyer Brain Cells

Format: PDF

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Cell Apps Poster Primary Cells

Format: PDF

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Cell Applications Inc Brochure

Format: PDF

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