Product Sheet CC10013
Description
BACKGROUND Structural maintenance of chromosomes (SMC) proteins fulfill pivotal roles in chromosome dynamics. In somatic cells, the heterodimeric Structural Maintenance of Chromosomes (SMC) proteins are involved in chromosome condensation and gene dosage compensation (SMC2 and 4), and sister chromatid cohesion and DNA recombination (SMC1 and 3). It was demonstrated that mammalian SMC1 and SMC3 proteins are also involved in meiosis.1 SMC1 and SMC3 proteins are important components of the cohesin complex, necessaryfor meiotic sister chromatid cohesion and DNA recombination . Mitotic chromosome segregation is facilitated by the cohesin complex, which maintains physical connections between sister chromatids until anaphase. Meiotic cell division is considerably more complex, as cohesion must be released sequentially to facilitate orderly segregation of chromosomes at both meiosis I and meiosis II. This necessitates meiosis-specific cohesin components; recent studies in rodents suggest that these influence chromosome behavior during both cell division and meiotic prophase. SMC1 is likely involved in maintaining cohesion between sister centromeres until anaphase II.2 In addition, it was also demonstrated that SMC1 is a component of the DNA damage response network that functions as an effector in the ATM/NBS1-dependent S-phase checkpoint pathway.3
REFERENCES
1. Eijpe M et al.: J. Cell Sc. 113:673-682, 2000.
2. Revenkova E et al.: Mol. Cell. Biol. 21:6984-6998, 2001.
3. Yazdi P T et al.: Genes Dev. 16:571–582, 2002.
2. Revenkova E et al.: Mol. Cell. Biol. 21:6984-6998, 2001.
3. Yazdi P T et al.: Genes Dev. 16:571–582, 2002.
Products are for research use only. They are not intended for human, animal, or diagnostic applications.
(Click to Enlarge) Top: Western Blot detection of endogenous SMC1 proteins from various normal primary cell lysates. Middle, upper: Immunohistochemical staining of paraffin-embedded colon cancer tissue, using Anti-SMC1. Middle, lower: It also stains NIH3T3 cells in confocal immunofluorescent testing (SMC1 Antibody: green; Actin filaments have been labeled with Alexa Fluor-555 phalloidin: Red). Bottom: It also reacts with HeLa cells in FACS assay (SMC-1 Antibody: green: control: purple).
Details
Cat.No.: | CC10013 |
Antigen: | Purified recombinant His-tagged fusion protein containing human SMC1 sequence (136-384 aa) expressed in E. Coli. |
Isotype: | Mouse Monoclonal IgG1 |
Species & predicted species cross- reactivity ( ): | Human, Rat, Mouse |
Applications & Suggested starting dilutions: | WB 1:1000 IP 1:50 IHC 1:50 - 1:200 IF 1:100 FACS 1:50 - 1:200 |
Predicted Molecular Weight of protein: | 150 kDa |
Specificity/Sensitivity: | This monoclonal antibody detects endogenous levels of SMC1 proteins in various normal primary cell lysates. |
Storage: | Store at -20°C, 4°C for frequent use. Avoid repeated freeze-thaw cycles. |
*Optimal working dilutions must be determined by end user.
Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Monoclonal Structural Maintenance of Chromosomes 1 Antibody: Monoclonal Structural Maintenance of Chromosomes 1 Antibody | Size: 100 ul | CAT.#: CC10013 | Price: $333.00 |