Product Sheet RP1201
Product Sheet RP1201AF
Description
BACKGROUND Fibroblast growth factor 6 (FGF-6) is a heparin-binding growth factor that is expressed in epithelial and mesenchymal lineages. FGF-6 binds and signals through the FGF receptors FGFR1, FGFR2, and FGFR4. FGF-6 functions as a mitogen for vascular endothelial cells and fibroblasts. FGF-6 is also an important factor driving muscle differentiation and regeneration.
Recombinant human Fibroblast growth factor 6 is a non-glycosylated protein monomer, containing 169 amino acids and having a molecular mass of 18 kDa.
Products are for research use only. They are not intended for human, animal, or diagnostic applications.
Details
Cat No.:
RP1201 / RP1201AF
Alternate Names:
Heparin secretory-transforming protein 2, HST-2, HSTF-2, Heparin-binding growth factor 6, HBGF-6
Source:
E. coli
Physical Appearance:
Sterile Filtered white lyophilized (freeze-dried) powder.
Formulation:
10 mM sodium phosphate and 50 mM sodium chloride, pH 7.5
Stability:
Lyophilized product is very stable at -20°C. Reconstituted material should be aliquoted and frozen at -20°C. It is recommended that a carrier protein (0.1% HSA or BSA) is added for long term storage.
Reconstitution:
Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at 0.1 mg/mL, which can be further diluted into other aqueous solutions.
Protein Content and Purity determined by:
• UV spectroscopy at 280 nm.
• RP-HPLC calibrated against a known standard.
• Quantitation against a known standard via reducing and non-reducing SDS-PAGE gels.
Endotoxin Level:
Endotoxin level, as measured by LAL analysis, is <0.01ng/ug or <0.1EU/ug.
Biological Activity:
The activity is measured by the dose-dependent NR6R-3T3 Proliferation w 1 ug heparin, with Bioactivity Acceptance Criteria ED50 at 1 ng/mL.
AA Sequence:
MGTRANNTLL DSRGWGTLLS RSRAGLAGEI AGVNWESGYL VGIKRQRRLY CNVGIGFHLQ VLPDGRISGT HEENPYSLLE ISTVERGVVS LFGVRSALFV AMNSKGRLYA TPSFQEECKF RETLLPNNYN AYESDLYQGT YIALSKYGRV KRGSKVSPIM TVTHFLPRI
Animal Component-Free
This product is produced with no animal derived raw products. All processing and handling employs animal free equipment and animal free protocols.
Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Human Fibroblast Growth Factor 6 (FGF-6): Human Fibroblast Growth Factor-6 | Size: 25 ug | CAT.#: RP1201-25 | Price: $195.00 | |
| Human Fibroblast Growth Factor 6 (FGF-6): Human Fibroblast Growth Factor-6 | Size: 100 ug | CAT.#: RP1201-100 | Price: $529.00 | |
| Human Fibroblast Growth Factor 6 (FGF-6): Human Fibroblast Growth Factor-6 | Size: 1000 ug | CAT.#: RP1201-1000 | Price: $3,173.00 | |
| Human FGF-6, Animal-Free: Human Fibroblast Growth Factor-6, Animal-Free | Size: 25 ug | CAT.#: RP1201AF-25 | Price: $85.00 | |
| Human FGF-6, Animal-Free: Human Fibroblast Growth Factor-6, Animal-Free | Size: 100 ug | CAT.#: RP1201AF-100 | Price: $582.00 | |
| Human FGF-6, Animal-Free: Human Fibroblast Growth Factor-6, Animal-Free | Size: 1000 ug | CAT.#: RP1201AF-1000 | Price: $2,327.00 |