Product Sheet RP2072
Product Sheet RP2072AF
Description
BACKGROUND Indian hedgehog (IHH) is an essential signaling factor that is secreted in the gut, cartilage, and bone during embryonic development. IHH acts through the patched (PTC) receptor to induce transcriptional changes important for bone and cartilage development. IHH also induces the expression of parathyroid hormone-related peptide (PTHrP), which in turn mediates IHH activity during chondrocyte differentiation, forming a negative feedback loop.
Recombinant Mouse Indian hedgehog is a non-glycosylated protein monomer, containing 177 amino acids and having a molecular mass of 19.9 kDa.
Products are for research use only. They are not intended for human, animal, or diagnostic applications.
Details
Cat. No.:
RP2073 / RP2073AF
Alternate Names:
HHG-2
Source:
E. coli
Physical Appearance:
Sterile Filtered white lyophilized (freeze-dried) powder.
Formulation:
0.1% Trifluoroacetic Acid (TFA)
Stability:
Lyophilized product is very stable at -20°C. Reconstituted material should be aliquoted and frozen at -20°C. It is recommended that a carrier protein (0.1% HSA or BSA) is added for long term storage.
Reconstitution:
Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product at 0.1 mg/ml in sterile 10 mM HCl, which can be further diluted into other aqueous solutions.
Protein Content and Purity determined by:
• UV spectroscopy at 280 nm.
• RP-HPLC calibrated against a known standard.
• Quantitation against a known standard via reducing and non-reducing SDS-PAGE gels.
Endotoxin Level:
Endotoxin level, as measured by LAL analysis, is <0.01ng/ug or <0.1EU/ug.
AA Sequence:
MIIGPGRVVG SRRRPPRKLV PLAYKQFSPN VPEKTLGASG RYEGKIARSS ERFKELTPNY NPDIIFKDEE NTGADRLMTQRCKDRLNSLA ISVMNQWPGV KLRVTEGWDE DGHHSEESLH YEGRAVDITT SDRDRNKYGL LARLAVEAGF DWVYYESKAH VHCSVKSEHSAAAKTGG
Animal Component-Free
This product is produced with no animal derived raw products. All processing and handling employs animal free equipment and animal free protocols.
Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Mouse Indian Iedgehog (IHH): Mouse Indian Hedgehog | Size: 25 ug | CAT.#: RP2073-25 | Price: $195.00 | |
| Mouse Indian Iedgehog (IHH): Mouse Indian Hedgehog | Size: 100 ug | CAT.#: RP2073-100 | Price: $441.00 | |
| Mouse Indian Iedgehog (IHH): Mouse Indian Hedgehog | Size: 1000 ug | CAT.#: RP2073-1000 | Price: $2,938.00 | |
| Mouse IHH, Animal-Free: Mouse IHH, Animal-Free | Size: 25 ug | CAT.#: RP2073AF-25 | Price: $215.00 | |
| Mouse IHH, Animal-Free: Mouse IHH, Animal-Free | Size: 100 ug | CAT.#: RP2073AF-100 | Price: $488.00 | |
| Mouse IHH, Animal-Free: Mouse IHH, Animal-Free | Size: 1000 ug | CAT.#: RP2073AF-1000 | Price: $3,231.00 |