Product Sheet RP1162
Product Sheet RP1162AF
Description
BACKGROUND Epstein-Barr virus induced gene-3 (EBI3), is a secreted glycoprotein belonging to the hematopoietin receptor family related to the p40 subunit of interleukin 12 (IL-12). EBI3 expression is induced in B-lymphocytes in response to Epstein-Barr virus infection. EBI3 forms heterodimers with p28 to form interleukin 27 (IL-27), and with p35 to form interleukin 35 (IL-35). Both IL-27 and IL-35 have anti-inflammatory and regulatory activity.
Recombinant human Epstein-Barr virus induced gene-3 is a non-glycosylated protein monomer, containing 210 amino acids and having a molecular mass of 23.4 kDa.
Products are for research use only. They are not intended for human, animal, or diagnostic applications.
Details
Cat. No.:
RP1162 / RP1162AF
Alternative Name:
IL-35/EBI3, IL-27/EBI3
Source:
E. coli
Physical Appearance:
Sterile Filtered white lyophilized (freeze-dried) powder.
Formulation:
Recombinant human EBI3 lyophilized with no additives.
Stability:
Lyophilized product is very stable at -20°C. Reconstituted material should be aliquoted and frozen at -20°C. It is recommended that a carrier protein (0.1% HSA or BSA) is added for long term storage.
Reconstitution:
Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 mg/ml, which can be further diluted into other aqueous solutions.
Protein Content and Purity determined by:
UV spectroscopy at 280 nm.
RP-HPLC calibrated against a known standard.
Quantitation against a known standard via reducing and non-reducing SDS-PAGE gels.
RP-HPLC calibrated against a known standard.
Quantitation against a known standard via reducing and non-reducing SDS-PAGE gels.
Endotoxin Level:
Endotoxin level, as measured by LAL analysis, is <0.01ng/ug or <0.1EU/ug.
Biological Activity:
Recombinant human EBI3 is determined by qualitative binding to an anti-EBI3 antibody.
AA Sequence:
MRKGPPAALT LPRVQCRASR YPIAVDCSWT LPPAPNSTSP VSFIATYRLG MAARGHSWPC LQQTPTSTSC TITDVQLFSM APYVLNVTAV HPWGSSSSFV PFITEHIIKP DPPEGVRLSP LAERQLQVQW EPPGSWPFPE IFSLKYWIRY KRQGAARFHR VGPIEATSFI LRAVRPRARY YVQVAAQDLT DYGELSDWSL PATATMSLGK
Animal Component-Free
This product is produced with no animal derived raw products. All processing and handling employs animal free equipment and animal free protocols.
Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Human Epstein-Barr Virus Induced Gene-3 (EBI3 / IL-27 / IL-35): Human EBV Induced Gene-3 | Size: 10 ug | CAT.#: RP1162-10 | Price: $194.00 | |
| Human Epstein-Barr Virus Induced Gene-3 (EBI3 / IL-27 / IL-35): Human EBV Induced Gene-3 | Size: 100 ug | CAT.#: RP1162-100 | Price: $624.00 | |
| Human Epstein-Barr Virus Induced Gene-3 (EBI3 / IL-27 / IL-35): Human EBV Induced Gene-3 | Size: 1000 ug | CAT.#: RP1162-1000 | Price: $5,327.00 | |
| Human EBI-3, Animal-Free: Human Epstein-Barr Virus Induced Gene-3, Animal-Free | Size: 10 ug | CAT.#: RP1162AF-10 | Price: $213.00 | |
| Human EBI-3, Animal-Free: Human Epstein-Barr Virus Induced Gene-3, Animal-Free | Size: 100 ug | CAT.#: RP1162AF-100 | Price: $687.00 | |
| Human EBI-3, Animal-Free: Human Epstein-Barr Virus Induced Gene-3, Animal-Free | Size: 1000 ug | CAT.#: RP1162AF-1000 | Price: $5,860.00 |