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Antiβ-Actin: Beta-Actin Mouse Monoclonal Antibody

Mouse Beta-Actin Antibody: Beta-Actin Mouse Monoclonal Antibody

Size: 100 ul

CAT. #: CC10028

Price: $302.00

Quantity

Description

BACKGROUND Actin is a ubiquitous globular protein that is one of the most highly-conserved proteins known. It is found in two main states; G-actin is the globular monomeric form, whereas F-actin forms helical polymers. Both G- and F-actin are intrinsically flexible structures – a feature vital in actin’s role as a dynamic filament network. Actin has four major functions. Firstly, F-actin polymers form microfilaments – polar intracellular ‘tracks’ for kinesin motor proteins, allowing the transport of vesicles, organelles and other cargo. Actin is a component of the cytoskeleton and links to alpha-actinin, E-cadherin and beta-catenin at adherens junctions. This gives mechanical support to cells and attaches them to each other and the extracellular matrix. In muscle cells, actin-rich thin filaments associate with myosin-rich thick filaments to form actomyosin myofibrils. Using energy from the hydrolysis of ATP, myofibrils undergo cyclic shortening through actin-myosin head interactions, which represents the mechanics of muscle contraction. Finally, actin has a role in cell motility through polymerization and depolymerization of fibrils.1 Beta actin, also known as cytoplasmic actin is a 42 kDa, highly conserved protein, ubiquitously expressed in all eukaryotic cells. It is a relatively stable cytoskeletal protein normally at a constant level in cells, regardless of experimental treatment or technical procedure.2 For this reason, measurement of beta-Actin is generally used as an internal control for experimental error.   

REFERENCES  

1. Pollard, D.T. & Cooper, J.A.: Science 326:1208-1212, 2009 2. Mori, R. et al: Prostate 68:1555-60, 2008    

Products are for research use only. They are not intended for human, animal, or diagnostic applications.

Details

Cat.No.:
CC10028
Antigen:
Raised against a short peptide from human beta-Actin sequence.
Isotype:
Mouse IgG1
Species & predicted
species cross-
reactivity ( ):
Human, Mouse, Rat
Applications &
Suggested starting
dilutions:*
WB                  1:1000
IP                    n/d
IHC                  n/d
ICC                  n/d
FACS               n/d
Predicted Molecular
Weight of protein:
42 kDa
Specificity/Sensitivity:
Detects endogenous beta-actin proteins without cross-reactivity with other family members.
Storage:
Store at -20°C, 4°C for frequent use. Avoid repeated freeze-thaw cycles.

*Optimal working dilutions must be determined by end user.

Resources

Product Sheet CC10028

Format: PDF

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