Anti-AQP2: Polyclonal Aquaporin 2 Antibody

AQP2 is expressed in the principal cells of the collecting ducts in the kidney and plays a critical role in the urine concentration. A unique feature of AQP2 is that it is stored in the intracellular compartment, and upon stimulation of an antidiuretic hormone (ADH, vasopressin), AQP2 translocates to the apical plasma membrane, where it serves in the uptake of water from the lumen of the collecting duct. It has been shown that AQP2 vesicles constitute a distinct intracellular compartment mostly in the apical cytoplasm and partially overlapping with early endosomes in collecting duct cells in the rat kidney. The AQP2 compartment is distinct from lysosomes, trans-Golgi network (TGN), Golgi apparatus, and endoplasmic reticulum. Numerous studies have focused on AQP2 trafficking from the intracellular compartment to the apical plasma membrane in response to ADH, and a signaling pathway via phosphorylation by protein kinase A and protein kinase G has been proposed. Moreover, its retrieval may use the apical endosomal system and the phosphatidylinositol 3-kinase-dependent pathway.² In addition, the mutation of AQP2 leads to nephrogenic diabetes insipidus, the inability to concentrate urine.³