Top: Western blot detection of c-Cbl proteins in various cell lysates using c-Cbl Antibody. Middle, Upper: It also stains paraffin-embedded human ovarian cancer tissue in IHC analysis. Middle, Lower: Thi
...Anti-c-Cbl: Mouse c-Cbl Antibody
Mouse c-Cbl Antibody: Mouse c-Cbl Antibody
Size: 100 ul
Price: $457.00
Description
BACKGROUNDÂ Cbl has emerged as a novel signal transducing protein downstream of a number of cell surface receptors coupled to tyrosine kinases. Identified as the protein product of the c-Cbl proto-oncogene, the cellular homolog to the transforming gene of a murine retrovirus, Cbl comprises an N-terminal transforming region (Cbl-N), which contains a phosphotyrosine binding (PTB) domain, and a C-terminal modular region (Cbl-C) containing a RING finger motif, a large proline-rich region and a leucine zipper. Deletion of Cbl-C or small deletions N-terminal to the RING finger render Cbl oncogenic, whereas wild type Cbl is non-transforming, even if overexpressed.1 Cbl serves as a substrate of both receptor and non-receptor tyrosine kinases, and binds to adaptor proteins Grb2, Crk and the p85 subunit of PI-3-kinase and mediates the downstream signaling.2 On the other hand, acting as an E3 ubiquitin-protein ligase, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and then transfers it to substrates promoting their degradation by the proteasome, Cbl also regulates receptor protein-tyrosine kinase ubiquitination in a manner dependent upon its variant SH2 and RING finger domains. Ubiquitination of receptor protein-tyrosine kinases terminates signaling by marking active receptors for degradation.3
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REFERENCESÂ Â
1. Lupher, M.L. Jr. et al: Int. J. Biochem. Cell Biol. 30:439-44, 1998
2. Smit, L. & Borst, J.:Crit. Rev. Oncog. 8:359-79, 1997
3. Thien, C.B.F. & Langdon, W.Y.: Biochem. J. 391:153-166, 2005
2. Smit, L. & Borst, J.:Crit. Rev. Oncog. 8:359-79, 1997
3. Thien, C.B.F. & Langdon, W.Y.: Biochem. J. 391:153-166, 2005
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Products are for research use only. They are not intended for human, animal, or diagnostic applications.
Details
| Cat.No.: | CP10401 |
| Antigen: | Raised against recombinant human c-Cbl fragments expressed in E. coli. |
| Isotype: | Mouse IgG1 |
| Species & predicted species cross- reactivity ( ): | Human, Mouse, Rat |
| Applications & Suggested starting dilutions:* | WBÂ Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â 1:1000 IPÂ Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â 1:50 IHCÂ Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â 1:50 – 1:200 ICCÂ Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â 1:50 – 1:200 FACSÂ Â Â Â Â Â Â Â Â Â Â Â Â Â 1:50 – 1:200 |
| Predicted Molecular Weight of protein: | 120 kDa |
| Specificity/Sensitivity: | Detects c-Cbl proteins in various cell lysate. |
| Storage: | Store at -20°C, 4°C for frequent use. Avoid repeated freeze-thaw cycles. |
*Optimal working dilutions must be determined by end user.