Top: Western Blot detection of CDC2/CDK1 proteins in Jurkat cell lysate using CDC2/CDK1 Antibody. Middle: This antibody stains HeLa cells in confocal immunofluorescent analysis (CDC2/CDK1 Antibody: Green; Actin filaments: Red; DRAQ5 DNA Dye: Blue).
Bottom: It also specifically reacts with CDC2/CDK1 proteins in PC-2 cells by
...Anti-CDC2/CDK1: Mouse CDC2/CDK1 Antibody
Mouse CDC2/CDK1 Antibody: Mouse CDC2/CDK1 Antibody
Size: 100 ul
Price: $413.00
Description
One important mechanism regulating the activity of CDC2 during the cell cycle is based on phosphorylation/ dephosphorylation. Avian CDC2 is phosphorylated Thr14, Tyr15, Thr161 and Ser277. Dephosphorylation of both Thr14 and Tyr15 is required for activation of CDC2 at the G2/M transition, indicating that phosphorylation of these residues negatively regulates CDC2 activity. Conversely, phosphorylation of Thr161 is required for kinase activity.3
2. Cisek, S.J. & Gorden, J.L. : Nature 339:679-84, 1989
3. Nigg, E.A. et al:Ciba Found. Symp. 170:72-84, 1992
Details
Cat.No.: | CP10295 |
Antigen: | Raised against recombinant human CDC2/CDK1 fragments expressed in E. coli. |
Isotype: | Mouse IgG1 |
Species & predicted species cross- reactivity ( ): | Human, Mouse, Rat |
Applications & Suggested starting dilutions:* | WB 1:1000 IP n/d IHC n/d ICC 1:50 – 1:200 FACS 1:50 – 1:200 |
Predicted Molecular Weight of protein: | 34 kDa |
Specificity/Sensitivity: | Detects endogenous CDC2/CDK1 proteins without cross-reactivity with other family members. |
Storage: | Store at -20°C, 4°C for frequent use. Avoid repeated freeze-thaw cycles. |
*Optimal working dilutions must be determined by end user.