“Alizarin Red Staining Solution” has been added to your cart. View cart
Top: Western blot detection of FGG proteins in human plasma sample using FGG Antibody. Bottom: This antibody stains NIH3T3 cells in confocal immunofluorescent testing (FGG antibody: Green; Actin filaments: Red; DRAQ5 DNA Dye: Blue).
Anti-FGG: Mouse Fibrinogen-gamma Antibody
Mouse Fibrinogen-Gamma Antibody: Mouse Fibrinogen-gamma Antibody
Size: 100 ul
Price: $457.00
Description
BACKGROUND Fibrinogen gamma chain, also known as FGG, is a human gene found on Chromosome 4. The protein encoded by this gene is the gamma component of fibrinogen, a blood-borne glycoprotein composed of three pairs of nonidentical polypeptide chains. Following vascular injury, fibrinogen is cleaved by thrombin to form fibrin which is the most abundant component of blood clots.1 In addition, various cleavage products of fibrinogen and fibrin regulate cell adhesion and spreading, display vasoconstrictor and chemotactic activities, and are mitogens for several cell types. Mutations in this gene lead to several disorders, including dysfibrinogenemia, hypofibrinogenemia and thrombophilia.2 Alternative splicing of the mRNA chain results in two transcript variants; the common γA chain and the alternatively spliced γ’ chain. Approximately 10% of the total plasma fibrinogen consists of γA/γ’ fibrinogen, with <1% consisting of γ'/γ' fibrinogen. Increased and decreased levels of γA/γ' fibrinogen have been associated with CAD and DVT respectively.
Â
REFERENCESÂ Â
1. Doolittle, R.F. et al: Annu. Rev. Biochem. 53: 195–229, 1984
2. Scott, E.M. et al: Arterioscler. Thromb. Vasc. Biol. 24: 1558–66, 2005
 Â
Products are for research use only. They are not intended for human, animal, or diagnostic applications.
Details
| Cat.No.: | CP10404 |
| Antigen: | Raised against recombinant human FGG fragments expressed in E. coli. |
| Isotype: | Mouse IgG1 |
| Species & predicted species cross- reactivity ( ): | Human, Mouse, Rat |
| Applications & Suggested starting dilutions:* | WBÂ Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â 1:1000 IPÂ Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â 1:50 IHCÂ Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â n/d ICCÂ Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â 1:50 – 1:200 FACSÂ Â Â Â Â Â Â Â Â Â Â Â Â Â n/d |
| Predicted Molecular Weight of protein: | 52 kDa |
| Specificity/Sensitivity: | Detects FGG proteins in various cell lysate. |
| Storage: | Store at -20°C, 4°C for frequent use. Avoid repeated freeze-thaw cycles. |
*Optimal working dilutions must be determined by end user.