Anti-HMGB-4: Rabbit High-Mobility-Group-Box 4 Antibody
Rabbit High-Mobility-Group-Box 4 Antibody: Rabbit High-Mobility-Group-Box 4 Antibody
Size: 100 ul
Price: $375.00
Description
HMGB4 is strongly and preferentially expressed in the adult mouse testis and weakly in the brain, but not in many other tissues. HMGB4 associates with chromatin, and in transfection assays, in contrast to HMGB1, it acts as a potent transcriptional repressor. During spermatogenesis, HMGB4 is present in the euchromatin of late pachytene spermatocytes and haploid round spermatids, whereas stronger expression is observed during the elongation phase, where it localizes to the basal pole of the nucleus in a manner mutually exclusive with H1FNT (H1T2) localized at the apical pole. HMGB4 basal localization is lost in H1FNT-mutant spermatids, showing that H1FNT provides a positional cue for organizing chromatin domains within the nucleus. These results show that HMGB4 and H1FNT specify distinct chromatin domains at the apical and basal poles of the elongating spermatid nucleus.3
2. Stros, M.: Biochim. Biophy. Acta 1799:101-13, 2010
3. Catena, R. et al: Biol. Reproduct. 80:385-66, 2009Â
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Details
Cat.No.: | CA1042 |
Antigen: | Short peptide from human HMGB-4 sequence. |
Isotype: | Rabbit IgG |
Species & predicted species cross- reactivity ( ): | Human, Rat |
Applications & Suggested starting dilutions:* | WBÂ Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â 1:1000 IPÂ Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â n/d IHCÂ Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â n/d ICCÂ Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â n/d FACSÂ Â Â Â Â Â Â Â Â Â Â Â Â Â n/d |
Predicted Molecular Weight of protein: | 22 kDa |
Specificity/Sensitivity: | Detects endogenous levels of HMGB-4 proteins without cross-reactivity with other related proteins. |
Storage: | Store at -20°C, 4°C for frequent use. Avoid repeated freeze-thaw cycles. |
*Optimal working dilutions must be determined by end user.