Anti-IL-8: Mouse Interleukin-8 Antibody

Interleukin (IL)-8, an approximately 8.5 kDa protein, a member of the CXC chemokine superfamily and is produced by a wide array of cell types, including epithelial cells, neutrophils, and macrophages in response to microbial infections. In healthy tissues, IL-8 is barely detectable, but is rapidly induced by nuclear factor kappaB (NF-kappaB) and activator protein 1 (AP-1)-dependent inflammatory stimuli such as TNFalpha, bacteria or virus. Neutrophil are major specific target for IL-8 action. This allows neutrophil to eradicate the invading pathogen within the site of infection. However, many pathophysiological actions of IL-8 also depend on activation of neutrophils. IL-8 is in routine use as a marker for various clinical conditions. In addition to be a potent chemoattractant and activator for neutrophils, T-cells, basophils, and eosinophils, IL-8 was alsoshown to promote the movement of cells of different lineages, such as fibroblasts and keratinocytes. Moreover, it was shown that IL-8 induced the migration of tumor cells and its expression was correlated with tumor growth, angiogenesis and metastatic potential in various human carcinomas and animal models.³ It was suggested that constitutive expression of IL-8 in tumor cell lines is associated with the metastatic potential and that IL-8 might act as an autocrine/paracrine growth factor in colon cancer progression and metastasis. The activities of IL-8 are mediated through its binding to specific GTP-coupled, seven-helix transmembrane receptors: CXCRl and CXCR2 (also known as IL-8 receptors type A and type B, respectively).⁴ IL-8 also binds to a receptor on erythrocytes, DARC, which binds a number of both CXC and CC chemokines.