Anti-Lyn: Mouse Lyn Antibody
Mouse Lyn Antibody: Mouse Lyn Antibody
Size: 100 ul
Price: $472.00
Description
Lyn, one of several Src-family tyrosine kinases in immune cells, has two isoforms p53Lyn and p56Lyn, resulting from alternative splicing of Lyn mRNA. It is preferentially expressed in B cells as well as other hematopoietic cells, but not in T cells. Lyn is noted for its ability to negatively regulate signaling pathways through phosphorylation of inhibitory receptors, enzymes, and adaptors. Somewhat paradoxically, it is also a key mediator in several pathways of B cell activation, such as CD19 and CD180. Whether Lyn functions to promote or inhibit immune cell activation depends on the stimulus and the developmental state, meaning that the consequences of Lyn activity are context dependent. The importance of regulating Lyn activity is exemplified by the pathological conditions that develop in both lyn-/- and lyn gain-of-function mice (lyn up/up), including lethal antibody-mediated autoimmune diseases and myeloid neoplasia.3
2. Brickell, P. M. et al: Crit. Rev. Oncol. 3:401-11, 1993
3. Xu, Y. et al: Immunity 22:9-18, 2005
Details
Cat.No.: | CP10318 |
Antigen: | Raised against recombinant human Lyn fragments expressed in E. coli. |
Isotype: | Mouse IgG1 |
Species & predicted species cross- reactivity ( ): | Human, Mouse, Rat |
Applications & Suggested starting dilutions:* | WB 1:1000 IP 1:50 IHC n/d ICC n/d FACS n/d |
Predicted Molecular Weight of protein: | 53/56 kDa |
Specificity/Sensitivity: | Detects endogenous Lyn proteins without cross-reactivity with other family members. |
Storage: | Store at -20°C, 4°C for frequent use. Avoid repeated freeze-thaw cycles. |
*Optimal working dilutions must be determined by end user.