Anti-MLH1: Mouse MutL Homolog 1 Antibody
Mouse MutL Homolog 1 Antibody: Mouse MutL Homolog 1 Antibody
Size: 100 ul
Price: $413.00
Description
There are over 300 MLH1 germline mutations described all along the gene that cause hereditary non-polyposis colorectal cancer (HNPCC). These mutations are not present in any particular hotspot or zone of the gene and include either nucleotide substitutions (missense, nonsense or splicing errors) or insertions/deletions (gross or small). In most of these mutations the resulting protein is truncated. There are also founding mutations which account for a high proportion of the HNPCC tumors in some specific populations (for example there are two Finnish mutations that delete the exons 16 or 6). Some germline genetic changes have also been described in both exons and introns as non pathogenic. There are described some sporadic mismatch repair deficiency cases (sporadic MSI) with somatic MLH1 mutations, although most of them have MLH1 promoter hypermetilation.3
2. Lipkin, S.M. et al: Nature Genet. 31:385-90, 2002
3. Bronner, C.E. et al: Nature 368:258-61, 1994Ā
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Details
| Cat.No.: | CP10170 |
| Antigen: | Purified recombinant human MLH1 fragment expressed in E. coli. |
| Isotype: | MouseĀ IgG1 |
| Species & predicted species cross- reactivity ( ): | Human, Mouse, Rat |
| Applications & Suggested starting dilutions:* | WBĀ Ā Ā Ā Ā Ā Ā Ā Ā Ā Ā Ā Ā Ā Ā Ā Ā 1:1000 IPĀ Ā Ā Ā Ā Ā Ā Ā Ā Ā Ā Ā Ā Ā Ā Ā Ā Ā Ā n/d IHCĀ Ā Ā Ā Ā Ā Ā Ā Ā Ā Ā Ā Ā Ā Ā Ā Ā Ā 1:200 ICCĀ Ā Ā Ā Ā Ā Ā Ā Ā Ā Ā Ā Ā Ā Ā Ā Ā Ā 1:200 FACSĀ Ā Ā Ā Ā Ā Ā Ā Ā Ā Ā Ā Ā Ā n/d |
| Predicted Molecular Weight of protein: | 85Ā kDa |
| Specificity/Sensitivity: | Detects endogenous MLH1 proteins without cross-reactivity with other family members. |
| Storage: | Store at -20Ā°C, 4Ā°C for frequent use. Avoid repeated freeze-thaw cycles. |
*Optimal working dilutions must be determined by end user.