Anti-MOF: MOF Mouse Monoclonal Antibody
MOF Mouse Monoclonal Antibody: MOF Mouse Monoclonal Antibody
Size: 100 ul
Price: $302.00
Description
Several studies suggest that MOF exists in multiple complexes in mammalian cells. In addition to the MSL complex, which is the highly conserved counterpart to the Drosophila MSL complex, MOF was reported to interact with several proteins, including WDR5, a key component of the MLL family H3 K4 methyltransferase complexes. It was further demonstrated that MOF forms a stable complex with MLL and supports p53-dependent transcription activation in vitro. In addition to MOF-MSL complex, another MOF complex, MOF-MSL1v1, was reported. It was demonstrated that the activity of the MOF-MSL1v1 complex is significantly different from that of the MSL complex. While the two MOF complexes have indistinguishable activity on histone H4 K16, they differ dramatically in acetylating nonhistone substrates. We demonstrate that the MOF-MSL1v1 complex is exclusively required for acetylating transcription factor p53 and for the optimal transcription activation of p53 target genes both in vitro and in vivo. Functional distinction of these two MOF complexes is further supported by their different binding patterns along the transcribed genes as well as their functional and/or physical interactions with different histone methyltransferases.4
2. Kind, J. et al: Cell 133:813-28, 2008
3. Sharma, G.G. et al: Mol. Cell. Biol. 30:3582-95, 2010
4. Li, X. et al: Mol. Cell 36:290-301, 2009
Details
Cat.No.: | CC10023 |
Antigen: | Raised against recombinant human MOF fragments expressed in E. coli. |
Isotype: | Mouse IgG1 |
Species & predicted species cross- reactivity ( ): | Human, Mouse |
Applications & Suggested starting dilutions:* | WB 1:1000 IP 1:50 IHC 1:100 ICC n/d FACS n/d |
Predicted Molecular Weight of protein: | 60 kDa |
Specificity/Sensitivity: | Detects endogenous MOF proteins in various cell lysate. |
Storage: | Store at -20°C, 4°C for frequent use. Avoid repeated freeze-thaw cycles. |
*Optimal working dilutions must be determined by end user.