Anti-NFkB: Polyclonal Nuclear Factor-kappa B, p65, Antibody
Polyclonal Nuclear Factor-Kappa B, p65, Antibody: Polyclonal Nuclear Factor-kappa B, p65, Antibody
Size: 100 ul
Price: $375.00
Description
In addition to regulation of NF-kappa-B activity through removal of IkappaB from NF-kappa-B/I B complexes, NF-kappa-B activity is also regulated through modulation of its transcriptional function. Changes in NF-kappa-B transcriptional activity have been assigned to inducible phosphorylation of the p65 subunit at Ser276, Ser529, and Ser536 by a large variety of kinases in response to different stimuli.4 Additionally, NF-kappa-B -dependent transcription requires multiple coactivators possessing histone acetyltransferase activity: CREB binding protein (CBP) and its homolog p300, p300/CBP-associated factor (P/CAF), SRC-1/NcoA-1, and TIF-2/GRIP-1/NcoA-2. Importantly, recruitment of CBP is enhanced by phosphorylation by the catalytic subunit of PKA (PKAc) of p65 at Ser276. More recently, other findings demonstrated a role for histone deacetylases (HDACs) as well. The first evidence came from the demonstration that inhibition of HDAC activity by trichostatin A (TSA) increases NF-kappa-B-dependent gene expression. It was next shown that NF-kappa-B interacts with distinct HDAC isoforms to negatively regulate gene expression, presumably through the deacetylation of histones and/or nonhistone proteins. Importantly, the phosphorylation status of p65 determines whether it associates with CBP/p300 or HDAC-1, ensuring that only signal-induced NF-kappa-B entering the nucleus can activate transcription.5
2. Dai, R. et al: J. Immunol.179:1776-83, 2007
3. Moorthy, A.K. et al: EMBO J. 25:1945-56, 2006
4. Vermeulen, L. et al: Biochem. Pharmacol. 64:673-90, 2002
5. Zhong, H. et al: Mol. Cell 9:625-36, 2002
Details
Cat.No.: | CA0610 |
Antigen: | C- terminal sequence of human NFkB (p65) |
Isotype: | Affinity-Purified Rabbit Polyclonal IgG |
Species & predicted species cross- reactivity ( ): | Human, Rabbit, Rat, Mouse |
Applications & Suggested starting dilutions:* | WB 1:500 to 1:1000 IP n/d IHC (Paraffin) 1:50 to 1:200 ICC n/d FACS n/d |
Predicted Molecular Weight of protein: | 65 kDa |
Specificity/Sensitivity: | Detects endogenous NF-kappa-B (p65) proteins without cross-reactivity with other family members. |
Storage: | Store at 4° C for frequent use; at -20° C for at least one year. |
*Optimal working dilutions must be determined by end user.