Anti-pp32: Mouse pp32 Antibody
Mouse pp32 Antibody: Mouse pp32 Antibody
Size: 100 ul
Price: $295.00
Description
pp32 (also known as PHAPI, INHAT) is a nuclear protein found highly expressed in normal tissues in those cells capable of self-renewal and in neoplastic cells. pp32 has been shown to be a binding partner of multiple important proteins. Previous work demonstrated that pp32 is involved in: 1) stabilization of certain mRNAs bearing AU-rich elements (AREs) in the 5′ and 3′ untranslated regions (UTRs) via the interaction of pp32 with the RNA-binding protein HuR (ELAVL1); 2) the modification of histone acetylation through its role in the inhibitor of acetyl transferase complex (termed INHAT); and 3) the modulation of the cell cycle through its interaction with the phosphorylated form of Rb. 4) interaction with Ataxin-1, a protein mutated in the neurodegenerative disease spinocerebellar ataxia type I, suggesting a possible role of PHAPI in this type of neurodegeneration. 5) Inhibition of protein phosphatase 2A (PP2A), which is a crucial regulator for various physiological processes.2
The multiple biochemical activities of pp32 suggest that this protein is involved in diverse biological events. To date, the most defined biological function of pp32I is its role as a tumor suppressor. pp32 expression appears to be greatly decreased during pancreatic cancer progression. In a study of human non-small cell lung cancer, pp32 expression level correlated with improved outcome following chemotherapy. Functionally, overexpression of pp32 can inhibit oncogene-induced tumorigenesis. It was shown that pp32 expression level correlates with the sensitivity of cancer cells to Apaf-1-mediated apoptosis. The apoptotic activity of PHAPI is required for its tumor-suppressive function.3
2. Williams, T.K. et al: PLoS ONE 5:e15455, 2010
3. Pan, W. et al: J. Biol. Chem. 284:6946-54, 2009
Details
| Cat.No.: | CP10293 |
| Antigen: | Raised against recombinant human pp32 fragments expressed in E. coli. |
| Isotype: | Mouse IgG1 |
| Species & predicted species cross- reactivity ( ): | Human, Mouse, Rat |
| Applications & Suggested starting dilutions:* | WB 1:1000 IP n/d IHC 1:50 – 1:200 ICC n/d FACS n/d |
| Predicted Molecular Weight of protein: | 28 kDa |
| Specificity/Sensitivity: | Detects endogenous pp32 proteins without cross-reactivity with other family members. |
| Storage: | Store at -20°C, 4°C for frequent use. Avoid repeated freeze-thaw cycles. |
*Optimal working dilutions must be determined by end user.
