Anti-RB: Mouse Rb Antibody

RB is phosphorylated in a cell cycle-dependent manner by cyclin-dependent protein kinases (cdk’s). In quiescent and early G1 cells, RB exists in a predominantly unphosphorylated state. As cells progress toward S phase, RB becomes phosphorylated. Initial phosphorylation of RB ismost likely catalyzed by cdk4-cyclin D or cdk6-cyclin D complexes; thereafter, RB phosphorylation is maintained by other cdk-cyclins. However, it was shown that activated ERK1/2 is also responsible for mitogen induced rapid Rb phosphorylation specifically at Ser780 and Ser795. This process occurs independent of cyclin/cdk pathway.² Many other kinases may be also involved in Rb phosphorylation, such as PKC etc.³ During mitosis, RB is rapidly dephosphorylated by an anaphase-specific phosphatase. Sixteen potential sites for cdk-mediated phosphorylation (Ser/Thr-Pro Motifs, Thr5, Ser230, Ser249, Thr252, Thr356, Thr373, Ser567, Ser608, Ser612, Ser780, Ser788, Ser807, Ser811, Thr821, and Thr826) exist in RB, and 11 of these sites have been shown to be phosphorylated in vivo.⁴ Different cdk-cyclin complexes preferentially phosphorylate RB at distinct sites in vitro, suggesting that different cdk-cyclin complexes may exert distinct effects on RB function in vivo. Phosphorylation of RB inactivates its growth suppression activity. In general, agents which block RB phosphorylation arrest cell cycle progression in G1, while agents which stimulate RB phosphorylation promote cell cycle progression. Phosphorylation of RB is required for G1/S progression. It was shown that p16INK4, an inhibitor of cdk4-cdk6, inhibits the phosphorylation of RB and arrests only RB-positive cells in G1. The biological significance of RB phosphorylation and its impact on the ordered progression through G1/S are exemplified by the frequent mutations that affect this process in human tumors. For example, amplification of the cyclin D1 gene is observed in breast cancer and esophageal cancer cells, in which it is believed to promote RB phosphorylation. Likewise, p16INK4, which acts to prevent RB phosphorylation, is lost in a large fraction of human tumors, again leading to the deregulated phosphorylation of RB. Additionally, the distinct phosphorylation sites are responsible for regulating the different protein binding activities of RB through different mechanisms. It was shown that phosphorylation of Thr356, Thr373, Ser-780, Ser-795, Ser-807, Ser-811, and Thr-821 decreases the binding of Rb to E2F, which would permit endothelial cells to progress fromG1 to later stages of the cell cycle.^3,5 On the other hand, upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex.