CXCL1 ELISA Kit, Mouse

CXCL1 is also found to be an oncogene and has pleiotropic effects on cell proliferation, tumor angiogenesis, invasion, and metastasis. It is frequently overexpressed in many cancers, such as squamous cell carcinoma, melanoma, breast cancer, ovarian cancer, colorectal cancer, and prostate cancer. Consistent with this, ectopic expression of CXCL1 promotes low CXCL1-producing cells to proliferate and spontaneous metastasis in BALB/c mice. Conversely, abolition of CXCL1 via anti- CXCL1-neutralizing antibody leads to reduction of tumor growth with decreased microvessel formation. Furthermore, CXCL1 was found to be a potent mediator of tumor-associated angiogenesis in colorectal and breast cancers.³ Transcriptional analysis revealed that the CXCL1 promoter contains several cis-acting elements, including the ones recognized by nuclear factor kappaB (NF-kappaB), Sp1, and human CUT homeodomain protein/CCAAT displacement protein. Indeed, NF-kappaB activity is found to be necessary for CXCL1 expression in microvascular endothelial cells and breast cancer cells. Thus, CXCL1 is regarded as one of NF-kappaB target genes. Futhermore, it was demonstrated that CXCL1 is transactivated by endogenous mutant p53 and serves as a crucial determinant for mutant p53 gain of function.⁴