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IL-15: Mouse Interleukin-15

IL-15: Mouse Interleukin-15

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Description

BACKGROUND Interleukin 15 (IL-15) is a widely expressed pro-inflammatory cytokine related to IL-2. IL-15 promotes activation of T cells, neutrophils and macrophages and is critical to dendritic cell function in several model systems. IL-15 has been shown to play a role in several inflammatory disorders, including rheumatoid arthritis, psoriasis and pulmonary inflammatory diseases. Emerging data suggest that IL-15 may serve as a good therapeutic target, as there appears to be a beneficial effect of IL-15 neutralization in models of psoriasis and diabetes. Human IL-15 shows activity on mouse cells. Recombinant mouse IL-15 is a non-glycosylated protein, containing 115 amino acids and having a molecular mass of 13.3 kDa. Products are for research use only. They are not intended for human, animal, or diagnostic applications.

Details

Cat. No.:
RP2007
 
Alternative Name:
IL-T
 
Source:
E. coli
 
Physical Appearance:
Sterile Filtered white lyophilized (freeze-dried) powder.
 
Formulation:
Recombinant mouse IL-15 was lyophilized from a concentrated (1mg/ml) solution with 10mM sodium phosphophate buffer, pH 8.0.
 
Stability:
Lyophilized product is very stable at -20°C. Reconstituted material should be aliquoted and frozen at -20°C. It is recommended that a carrier protein (0.1% HSA or BSA) is added for long term storage.
 
Reconstitution:
Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 mg/ml, which can be further diluted into other aqueous solutions.
 
Protein Content and Purity determined by:
UV spectroscopy at 280 nm.
RP-HPLC calibrated against a known standard.
Quantitation against a known standard via reducing and non-reducing SDS-PAGE gels.
 
Endotoxin Level:
Endotoxin level, as measured by LAL analysis, is <0.01ng/ug or <0.1EU/ug.
 
Biological Activity:
The activity is determined by dose-dependent induction of CTLL-2 cell proliferation and is typically less than 0.2 ng/ml.
 
AA Sequence:
MNWIDVRYDL EKIESLIQSI HIDTTLYTDS DFHPSCKVTA MNCFLLELQV ILHEYSNMTL NETVRNVLYL ANSTLSSNKN VAESGCKECE ELEEKTFTEF LQSFIRIVQM FINTS
 

Resources

Product Sheet RP2007

Format: PDF

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