Skip to main content
IL-27 / p28: Mouse Interleukin-27 (p28 subunit)

IL-27 / p28: Mouse Interleukin-27 (p28 subunit)

Quantity
Add to quote
Add to quote

Description

BACKGROUND The p28 subunit of interleukin 27 (IL-27), also known as interleukin 30 (IL-30), is a member of the interleukin 12 (IL-12) family of cytokines.  p28 is a 28 kilodalton polypeptide that associates with the Epstein-Barr virus induced gene 3 (EBI3) to form the IL-27 cytokine heterodimer complex.  IL-27 functions as a proinflammatory cytokine that induces immunomodulatory effects in naïve CD4+ T cells, mast cells, and monocytes.  p28 can also form a complex with cytokine-like factor 1 (CLF), that is secreted by dendritic cells, to regulate natural killer (NK) and T cell function.

Recombinant mouse p28 subunit of interleukin 27 is a non-glycosylated protein monomer, containing 207 amino acids and having a molecular mass of 23.7 kDa.

Products are for research use only. They are not intended for human, animal, or diagnostic applications.

Details

Cat. No.:
RP2050 / RP2050AF
Alternative Name:
IL-30, P28, IL-27 p28 subunit
Source:
E. coli
Physical Appearance:
Sterile Filtered white lyophilized (freeze-dried) powder.
Formulation:
Recombinant mouse IL-27/p28 is lyophilized at a concentration of 1 mg/ml in 10 mM sodium phosphophate buffer, pH 7.5.
Stability:
Lyophilized product is very stable at -20°C. Reconstituted material should be aliquoted and frozen at -20°C. It is recommended that a carrier protein (0.1% HSA or BSA) is added for long term storage.
Reconstitution:
Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 mg/ml, which can be further diluted into other aqueous solutions.
Protein Content and Purity determined by:
UV spectroscopy at 280 nm.
RP-HPLC calibrated against a known standard.
Quantitation against a known standard via reducing and non-reducing SDS-PAGE gels.
Endotoxin Level:
Endotoxin level, as measured by LAL analysis, is <0.01ng/ug or <0.1EU/ug.
This product is produced with no animal derived raw products.  All processing and handling employs animal free equipment and animal free protocols.

Resources

Product Sheet RP2050

Format: PDF

DOWNLOAD NOW
Product Sheet RP2050AF

Format: PDF

DOWNLOAD NOW

Related products