 Tissue RNA.  Human Fetal Brain (lines 2, 3) and Human Adult Lung (lines 4, 5) Total RNA. RNA was electrophoresed in 1% agarose gel to show the distinct 28S and 18S ribosomal RNA bands (lines 2, 4). RT-PCR was performed with HSP60 primers to produce 2.2 kb product (lines 3, 5).
Tissue RNA
Price: $0.00
Description
Total RNA prepared from normal human tissues using the modified guanidium thiocyanate method. RNA is DNAse treated to remove contaminating genomic DNA and undergoes stringent quality control tests. Purified RNA is ready for use in convenient 50 & 250 µg sizes for various downstream applications, including microarray hybridization, PCR cDNA synthesis and RT-PCR analysis.
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See also Cell RNA
Details
Stringent quality control tests confirm RNA integrity and stability:
- Distinct 28S and 18S ribosomal RNA were shown on 1% denaturing gel before and after 37CÂ incubation
- No visible Genomic DNA band of 23 kb detected
- No genomic DNA contamination detected following PCR amplification using house-keeping gene G3PDH primers
- A 2.2 kb product from mRNA was detected following RT-PCR amplification using human heat shock gene Hsp60 primers
- A discrete band of 2 kb was detected upon hybridization with a human beta-actin cDNA probe in Northern Blot analysis
- Primers were designed to detect mRNA for genes of interest to confirm cell phenotype