TPO ELISA Kit, Mouse

c-Mpl belongs to the type I cytokine receptor family. Ligand binding induces activation of the JAK2 that is associated with the receptor through Box1 and Box2. Activated JAK2 phosphorylates tyrosine residues on cytokine receptors, thereby providing docking sites for several Src homology 2 (SH2) domain–containing downstream signaling proteins. Mpl activates many signaling pathways in hematopoietic cell lines and primary megakaryocytes and platelets, including STAT3 and 5, Shc–Ras–mitogen-activated protein kinase (MAPK), and SHP2–Gab–phosphoinositide-3 kinase–Akt pathways. On the other hand, Lnk, an adptor protein, negatively regulates thrombopoietin (TPO) signaling in megakaryocytes and erythropoietin signaling in erythroblasts. Thus, it is suggested that a balance in positive and negative signals downstream from the TPO signal plays a role in the regulation of the probability of self-renewal in hematopoietic stem cells. These signaling molecules have important roles in hematopoiesis, and dysregulation of these signaling pathways is implicated in leukemogenesis.³