Cytofect™ Transfection Reagents: Frequently Asked Questions (FAQs)
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The Cytofect™ Transfection Reagents are a set of unique reagents that complex with plasmid DNA and escort genes to the nucleus. The result is higher delivery efficiency and lower cytotoxicity compared to leading competitors’ reagents, especially in primary endothelial, epithelial, and fibroblast cells.
The Cytofect™ Trasfection Kits contains all necessary Cytofect™ reagents for optimal gene transfer, as well as media for the transfection procedure.
Please see the table below for transfection efficiencies in a variety of primary cells tested by Cell Applications:
| Cytofect™ Kit | Efficiency | Viability | Cell Type |
|---|---|---|---|
| Endothelial Cell | 40% – 85% | 80% – 95% | HUVEC HAOEC HCAEC HPAEC HLMVEC BAOEC BCAEC BPAEC RAOEC |
| HUVEC | 40% – 85% | 80% – 95% | HUVEC |
| Epithelial Cell | 40% – 70% | 70% – 95% | HBEpC HTEpC HMEpC HEK |
| Fibroblast | 30% – 70% | 90% – 95% | HDF HCF HLF |
| Smooth Muscle | 35%-65% | 80% – 95% | HAOSMC HBcASMC HCtASMC HCASMC HITASMC HPASMC HScASMC HUVSMC |
| Hepatocyte | 60% – 70% | 90% – 95% | RH |
| Cell Line | 40% – 90% | 80% – 95% | HEK 293 CHO HeLa MCF-7 |
We strongly recommend transfecting cells after at least one passage but no longer than three passages. Ideally, your cells should be transfected when they are at the peak of health, which is typically 70-80% confluency.
Typically, our DNA is diluted in sterile water at a concentration of 0.2 µg/µl
The Cytofect™ Transfection Kits have very low cytotoxicity compared to leading competitor’s transfection reagents and compared to electroporation. Please see the post-transfection viabilities shown on the chart from Question # 3 above.
The Cytofect™ Transfection Kits are stable for 1 year when stored properly.