Product Sheet RP1197
Product Sheet RP1197AF
Description
BACKGROUND WNT1-inducible-signaling pathway protein 2 (WISP-2) is a member of the CYR61/CTGF/NOV (CCN) family of regulatory factors. WISP-2 is expressed in ectodermal, mesodermal, and endodermal lineages, including primary osteoblasts, fibroblasts, mesenchymal stem cells, and adipogenic precursor cells. WISP-2 is a canonical WNT ligand that regulates cell proliferation, adhesion, and metastasis. Secreted WISP-2 promotes mesenchymal precursor cell proliferation and maintains them in an undifferentiated state. In bone-forming osteoblasts, WISP-2 promotes osteoblast adhesion and inhibits osteocalcin production.
Recombinant Human WNT1-Inducible Signaling Pathway Protein 2 is a non-glycosylated protein monomer, containing 228 amino acids and having a molecular mass of 24.4 kDa.
Products are for research use only. They are not intended for human, animal, or diagnostic applications.
Details
Cat. No.:
RP1197AF
Alternate Names:
CTGFL, Connective Tissue Growth Factor-Like protein, CCN5, CT-58
Source:
E. coli
Physical Appearance:
Sterile Filtered white lyophilized (freeze-dried) powder.
Formulation:
0.1% Trifluoroacetic Acid (TFA)
Stability:
Lyophilized product is very stable at -20°C. Reconstituted material should be aliquoted and frozen at -20°C. It is recommended that a carrier protein (0.1% HSA or BSA) is added for long term storage.
Reconstitution:
Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile 10 mM Aceitic acid at 0.1 mg/mL, which can be further diluted into other aqueous solutions.
Protein Content and Purity determined by:
• UV spectroscopy at 280 nm.
• RP-HPLC calibrated against a known standard.
• Quantitation against a known standard via reducing and non-reducing SDS-PAGE gels.
Endotoxin Level:
Endotoxin level, as measured by LAL analysis, is <0.01ng/ug or <0.1EU/ug.
AA Sequence:
MQLCPTPCTC PWPPPRCPLG VPLVLDGCGC CRVCARRLGE PCDQLHVCDA SQGLVCQPGA GPGGRGALCL LAEDDSSCEV NGRLYREGET FQPHCSIRCR CEDGGFTCVP LCSEDVRLPS WDCPHPRRVE VLGKCCPEWV CGQGGGLGTQ PLPAQGPQFS GLVSSLPPGV PCPEWSTAWG PCSTTCGLGM ATRVSNQNRF CRLETQRRLC LSRPCPPSRG RSPQNSAF
Animal Component-Free
This product is produced with no animal derived raw products. All processing and handling employs animal free equipment and animal free protocols.
Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Human WNT1-Inducible Signaling Pathway Protein 2 (WISP-2 / CTGF-L): Human WNT1-Inducible Signaling Pathway Protein 2 | Size: 20 ug | CAT.#: RP1197-20 | Price: $195.00 | |
| Human WNT1-Inducible Signaling Pathway Protein 2 (WISP-2 / CTGF-L): Human WNT1-Inducible Signaling Pathway Protein 2 | Size: 100 ug | CAT.#: RP1197-100 | Price: $682.00 | |
| Human WNT1-Inducible Signaling Pathway Protein 2 (WISP-2 / CTGF-L): Human WNT1-Inducible Signaling Pathway Protein 2 | Size: 1000 ug | CAT.#: RP1197-1000 | Price: $4,113.00 | |
| Human WISP-2, Animal-Free: Human WNT1-Inducible Signaling Pathway Protein 2, Animal-Free | Size: 20 ug | CAT.#: RP1197AF-20 | Price: $215.00 | |
| Human WISP-2, Animal-Free: Human WNT1-Inducible Signaling Pathway Protein 2, Animal-Free | Size: 100 ug | CAT.#: RP1197AF-100 | Price: $752.00 | |
| Human WISP-2, Animal-Free: Human WNT1-Inducible Signaling Pathway Protein 2, Animal-Free | Size: 1000 ug | CAT.#: RP1197AF-1000 | Price: $4,524.00 |