Cell RNA (A) Human FibroblastāLike Synoviocytes, Rheumatoid Arthritis (HFLS-RA) Total
...Cell RNA
Description
High quality total RNAĀ from human and animal primary cells using the modified guanidium thiocyanate method.Ā RNA is offered at 10 & 25 Ī¼g/vial at 1 mg/ml, and is ready for use in downstream applications like microarray hybridization, PCR cDNA synthesis and RT-PCR analysis.
See Also Tissue RNA
Details
Quality control tests confirm RNA integrity and stability:
- Distinct 28S and 18S ribosomal RNA shown on 1% denaturing gel before and after 37oCĀ incubation
- No visible Genomic DNA band of 23 kb detected
- No genomic DNA contamination detected following PCR amplification using house-keeping gene G3PDH primers
- A 2.2 kb product from mRNA was detected following RT-PCR amplification using human heat shock gene Hsp60 primers
- A discrete band of 2 kb was detected upon hybridization with a human beta-actin cDNA probe in Northern Blot analysis
- Primers were designed to detect mRNA for genes of interest to confirm cell phenotype